Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

被引：163

作者：

Armache, Jean-Paul ^{[1
,9
]}

Jarasch, Alexander ^{[1
,9
]}

Anger, Andreas M. ^{[1
,9
]}

Villa, Elizabeth ^{[2
]}

Becker, Thomas ^{[1
,9
]}

Bhushan, Shashi ^{[1
,9
]}

Jossinet, Fabrice ^{[3
]}

Habeck, Michael ^{[4
,5
]}

Dindar, Guelcin ^{[1
,9
]}

Franckenberg, Sibylle ^{[1
,9
]}

Marquez, Viter ^{[1
,9
]}

Mielke, Thorsten ^{[6
,7
]}

Thomm, Michael ^{[8
]}

Berninghausen, Otto ^{[1
,9
]}

Beatrix, Birgitta ^{[1
,9
]}

Soeding, Johannes ^{[1
,9
]}

Westhof, Eric ^{[3
]}

Wilson, Daniel N. ^{[1
,9
]}

Beckmann, Roland ^{[1
,9
]}

机构：

[1] Univ Munich, Dept Biochem, Gene Ctr, D-81377 Munich, Germany

[2] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany

[3] Univ Strasbourg, Inst Biol Mol & Cellulaire, CNRS, F-67084 Strasbourg, France

[4] Max Planck Inst Biol Cybernet, Dept Empir Inference, D-72076 Tubingen, Germany

[5] Max Planck Inst Dev Biol, Dept Prot Evolut, D-72076 Tubingen, Germany

[6] Max Planck Inst Mol Genet, UltraStrukturNetzwerk, D-14195 Berlin, Germany

[7] Charite, Inst Med Phys & Biophys, D-10117 Berlin, Germany

[8] Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany

[9] Univ Munich, Dept Biochem, Ctr Integrated Prot Sci Munich, D-81377 Munich, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 46期

关键词：

modeling; molecular dynamics; flexible fitting; 5.5 ANGSTROM RESOLUTION; ELECTRON-MICROSCOPY; MOLECULAR-DYNAMICS; MESSENGER-RNA; RAT-LIVER; SECONDARY STRUCTURE; EXPANSION SEGMENTS; CROSS-LINKING; 70S RIBOSOME; EXIT TUNNEL;

D O I：

10.1073/pnas.1009999107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution. This map, together with a 6.1-angstrom map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model similar to 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

引用

页码：19748 / 19753

页数：6

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