Electrons, Photons, and Force: Quantitative Single-Molecule Measurements from Physics to Biology

被引：83

作者：

Claridge, Shelley A. ^{[1
,2
]}

Schwartz, Jeffrey J. ^{[1
,3
]}

Weiss, Paul S. ^{[1
,2
,4
]}

机构：

[1] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Mat Sci & Engn, Los Angeles, CA 90095 USA

来源：

ACS NANO | 2011年 / 5卷 / 02期

关键词：

single molecule; scanning tunneling microscopy; atomic force microscopy; transmission electron microscopy; optical microscopy; subdiffraction microscopy; Forster resonance energy transfer; fluorescence; photoactivation light microscopy; magnetic resonance; spin; magnetic resonance force microscopy; electron spin resonance; nitrogen vacancy; SELF-ASSEMBLED MONOLAYERS; SCANNING-TUNNELING-MICROSCOPY; ENHANCED RAMAN-SCATTERING; RESONANCE ENERGY-TRANSFER; MAGNETIC-RESONANCE; HIGH-RESOLUTION; FLUORESCENCE SPECTROSCOPY; CONFORMATIONAL DYNAMICS; INTERMOLECULAR INTERACTIONS; ALKANETHIOL MONOLAYERS;

D O I：

10.1021/nn103298x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-molecule measurement techniques have illuminated unprecedented details of chemical behavior, including observations of the motion of a single molecule on a surface, and even the vibration of a single bond within a molecule. Such measurements are critical to our understanding of entities ranging from single atoms to the most complex protein assemblies. We provide an overview of the strikingly diverse classes of measurements that can be used to quantify single-molecule properties, including those of single macromolecules and single molecular assemblies, and discuss the quantitative insights they provide. Examples are drawn from across the single-molecule literature, ranging from ultrahigh vacuum scanning tunneling microscopy studies of adsorbate diffusion on surfaces to fluorescence studies of protein conformational changes in solution.

引用

页码：693 / 729

页数：37

共 338 条

[61] Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2 [J].