Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

被引:117
作者
Grigorieff, Nikolaus [1 ,4 ]
Harrison, Stephen C. [2 ,3 ,4 ]
机构
[1] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02154 USA
[2] Harvard Univ, Sch Med, Boston, MA 02115 USA
[3] Childrens Hosp, Boston, MA 02115 USA
[4] Howard Hughes Med Inst, Chevy Chase, MD USA
关键词
SINGLE-PARTICLE RECONSTRUCTIONS; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; ANGSTROM RESOLUTION; RADIATION-DAMAGE; CRYO-EM; IMAGE-RECONSTRUCTION; SPHERICAL VIRUSES; FOURIER SYNTHESIS; PHASE-CONTRAST;
D O I
10.1016/j.sbi.2011.01.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4 angstrom resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.
引用
收藏
页码:265 / 273
页数:9
相关论文
共 73 条
[31]   Automated determination of parameters describing power spectra of micrograph images in electron microscopy [J].
Huang, Z ;
Baldwin, PR ;
Mullapudi, S ;
Penczek, PA .
JOURNAL OF STRUCTURAL BIOLOGY, 2003, 144 (1-2) :79-94
[32]   Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy [J].
Jiang, Wen ;
Baker, Matthew L. ;
Jakana, Joanita ;
Weigele, Peter R. ;
King, Jonathan ;
Chiu, Wah .
NATURE, 2008, 451 (7182) :1130-U12
[33]  
Jones T.A., 1992, MOL REPLACEMENT
[34]  
Kleywegt G.J., 2001, INT TABLES CRYSTALLO
[35]   IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database [J].
Liang, YY ;
Ke, EY ;
Zhou, ZH .
JOURNAL OF STRUCTURAL BIOLOGY, 2002, 137 (03) :292-304
[36]   Structure of the reovirus membrane-penetration protein, μ1, in a complex with its protector protein, σ3 [J].
Liemann, S ;
Chandran, K ;
Baker, TS ;
Nibert, ML ;
Harrison, SC .
CELL, 2002, 108 (02) :283-295
[37]   Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions [J].
Liu, Hongrong ;
Cheng, Lingpeng ;
Zeng, Songjun ;
Cai, Canying ;
Zhou, Z. Hong ;
Yang, Qibin .
JOURNAL OF STRUCTURAL BIOLOGY, 2008, 161 (01) :64-73
[38]   Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks [J].
Liu, Hongrong ;
Jin, Lei ;
Koh, Sok Boon S. ;
Atanasov, Ivo ;
Schein, Stan ;
Wu, Lily ;
Zhou, Z. Hong .
SCIENCE, 2010, 329 (5995) :1038-1043
[39]   Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-path Simulated Annealing optimization algorithm [J].
Liu, Xiangan ;
Jiang, Wen ;
Jakana, Joanita ;
Chiu, Wah .
JOURNAL OF STRUCTURAL BIOLOGY, 2007, 160 (01) :11-27
[40]   Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus [J].
Liu, Xiangan ;
Zhang, Qinfen ;
Murata, Kazuyoshi ;
Baker, Matthew L. ;
Sullivan, Matthew B. ;
Fu, Caroline ;
Dougherty, Matthew T. ;
Schmid, Michael F. ;
Osburne, Marcia S. ;
Chisholm, Sallie W. ;
Chiu, Wah .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2010, 17 (07) :830-U76