Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

被引：117

作者：

Grigorieff, Nikolaus ^{[1
,4
]}

Harrison, Stephen C. ^{[2
,3
,4
]}

机构：

[1] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02154 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

[3] Childrens Hosp, Boston, MA 02115 USA

[4] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2011年 / 21卷 / 02期

关键词：

SINGLE-PARTICLE RECONSTRUCTIONS; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; ANGSTROM RESOLUTION; RADIATION-DAMAGE; CRYO-EM; IMAGE-RECONSTRUCTION; SPHERICAL VIRUSES; FOURIER SYNTHESIS; PHASE-CONTRAST;

D O I：

10.1016/j.sbi.2011.01.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4 angstrom resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.

引用

页码：265 / 273

页数：9

共 73 条

[41] EMAN: Semiautomated software for high-resolution single-particle reconstructions [J].

Ludtke, SJ ;

Baldwin, PR ;

Chiu, W .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 128 (01) :82-97

[42] Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate [J].

Majorovits, E. ;

Barton, B. ;

Schultheiss, K. ;

Perez-Willard, F. ;

Gerthsen, D. ;

Schroeder, R. R. .

ULTRAMICROSCOPY, 2007, 107 (2-3) :213-226

[43] X-ray Crystal Structure of the Rotavirus Inner Capsid Particle at 3.8 Å Resolution [J].

McClain, Brian ;

Settembre, Ethan ;

Temple, Brenda R. S. ;

Bellamy, A. Richard ;

Harrison, Stephen C. .

JOURNAL OF MOLECULAR BIOLOGY, 2010, 397 (02) :587-599

[44] Detective quantum efficiency of electron area detectors in electron microscopy [J].

McMullan, G. ;

Chen, S. ;

Henderson, R. ;

Faruqi, A. R. .

ULTRAMICROSCOPY, 2009, 109 (09) :1126-1143

[45] Active pixel sensor array as a detector for electron microscopy [J].

Milazzo, AC ;

Leblanc, P ;

Duttweiler, F ;

Jin, L ;

Bouwer, JC ;

Peltier, S ;

Ellisman, M ;

Bieser, F ;

Matis, HS ;

Wieman, H ;

Denes, P ;

Kleinfelder, S ;

Xuong, NH .

ULTRAMICROSCOPY, 2005, 104 (02) :152-159

[46] Accurate determination of local defocus and specimen tilt in electron microscopy [J].

Mindell, JA ;

Grigorieff, N .

JOURNAL OF STRUCTURAL BIOLOGY, 2003, 142 (03) :334-347

[47] Atomic model of the papillomavirus capsid [J].

Modis, Y ;

Trus, BL ;

Harrison, SC .

EMBO JOURNAL, 2002, 21 (18) :4754-4762

[48] Design of an electron microscope phase plate using a focused continuous-wave laser [J].

Mueller, H. ;

Jin, Jian ;

Danev, R. ;

Spence, J. ;

Padmore, H. ;

Glaeser, R. M. .

NEW JOURNAL OF PHYSICS, 2010, 12

[49] On the three-dimensional reconstruction of icosahedral particles [J].

Navaza, J .

JOURNAL OF STRUCTURAL BIOLOGY, 2003, 144 (1-2) :13-23

[50] FUNDAMENTALS OF THREE-DIMENSIONAL RECONSTRUCTION FROM PROJECTIONS [J].

Penczek, Pawel A. .

METHODS IN ENZYMOLOGY, VOL 482: CRYO-EM, PART B: 3-D RECONSTRUCTION, 2010, 482 :1-33

← 1 2 3 4 5 6 7 8 →