Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

被引：117

作者：

Grigorieff, Nikolaus ^{[1
,4
]}

Harrison, Stephen C. ^{[2
,3
,4
]}

机构：

[1] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02154 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

[3] Childrens Hosp, Boston, MA 02115 USA

[4] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2011年 / 21卷 / 02期

关键词：

SINGLE-PARTICLE RECONSTRUCTIONS; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; ANGSTROM RESOLUTION; RADIATION-DAMAGE; CRYO-EM; IMAGE-RECONSTRUCTION; SPHERICAL VIRUSES; FOURIER SYNTHESIS; PHASE-CONTRAST;

D O I：

10.1016/j.sbi.2011.01.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4 angstrom resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.

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收藏

页码：265 / 273

页数：9

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