A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

被引：18

作者：

Murata, T ^{[1
]}

Goshima, F ^{[1
]}

Koshizuka, T ^{[1
]}

Takakuwa, H ^{[1
]}

Nishiyama, Y ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Dis Mechanism & Control Res Inst, Virol Lab,Showa Ku, Nagoya, Aichi 4668550, Japan

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 02期

关键词：

D O I：

10.1128/JVI.75.2.1039-1043.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.

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页码：1039 / 1043

页数：5

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