Plant structure visualization by high-resolution X-ray computed tomography

被引:155
作者
Dhondt, Stijn [1 ,2 ]
Vanhaeren, Hannes [1 ,2 ]
Van Loo, Denis [3 ]
Cnudde, Veerle [4 ]
Inze, Dirk [1 ,2 ]
机构
[1] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium
[3] Univ Ghent, UGCT Dept Phys & Astron, B-9000 Ghent, Belgium
[4] Univ Ghent, UGCT Dept Geol & Soil Sci, B-9000 Ghent, Belgium
关键词
GENE-EXPRESSION; ARABIDOPSIS; CT; PHLOEM; TISSUE; WOOD;
D O I
10.1016/j.tplants.2010.05.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
New developments in high-resolution X-ray computed tomography (HRXCT) are promising for the broader application of this non-destructive imaging method in plant sciences. Here, we demonstrate how detailed three-dimensional morphological traits can be extracted rapidly from in vivo Arabidopsis thaliana seedlings without sample manipulation. Furthermore, ex vivo scanning at sub-micron resolution allows the quantification and visualization of the cellular organization of plant tissue samples, making HRXCT a desired tool in developmental plant biology.
引用
收藏
页码:419 / 422
页数:4
相关论文
共 23 条
[1]   Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network [J].
Cloetens, Peter ;
Mache, Regis ;
Schlenker, Michel ;
Lerbs-Mache, Silva .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (39) :14626-14630
[2]   Virtual histology by means of high-resolution X-ray CT [J].
Cnudde, V. ;
Masschaele, B. ;
De Cock, H. E. V. ;
Olstad, K. ;
Vlaminck, L. ;
Vlassenbroeck, J. ;
Dierick, M. ;
Witte, Y. D. ;
Van Hoorebeke, L. ;
Jacobs, P. .
JOURNAL OF MICROSCOPY, 2008, 232 (03) :476-485
[3]   Recent progress in X-ray CT as a geosciences tool [J].
Cnudde, V. ;
Masschaele, B. ;
Dierick, M. ;
Vlassenbroeck, J. ;
Van Hoorebeke, L. ;
Jacobs, P. .
APPLIED GEOCHEMISTRY, 2006, 21 (05) :826-832
[4]   Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis [J].
Ferreira, Stephanus J. ;
Senning, Melanie ;
Sonnewald, Sophia ;
Kessling, Petra-Maria ;
Goldstein, Ralf ;
Sonnewald, Uwe .
BMC GENOMICS, 2010, 11
[5]   Non-invasive time-lapsed monitoring and quantification of engineered bone-like tissue [J].
Hagenmueller, Henri ;
Hofmann, Sandra ;
Kohler, Thomas ;
Merkle, Hans P. ;
Kaplan, David L. ;
Vunjak-Novakovic, Gordana ;
Mueller, Ralph ;
Meinel, Lorenz .
ANNALS OF BIOMEDICAL ENGINEERING, 2007, 35 (10) :1657-1667
[6]  
Heeraman DA, 1997, PLANT SOIL, V189, P167, DOI 10.1023/A:1004258818538
[7]   Optical sectioning deep inside live embryos by selective plane illumination microscopy [J].
Huisken, J ;
Swoger, J ;
Del Bene, F ;
Wittbrodt, J ;
Stelzer, EHK .
SCIENCE, 2004, 305 (5686) :1007-1009
[8]   Combined MRI-PET dissects dynamic changes in plant structures and functions [J].
Jahnke, Siegfried ;
Menzel, Marion I. ;
van Dusschoten, Dagmar ;
Roeb, Gerhard W. ;
Buehler, Jonas ;
Minwuyelet, Senay ;
Bluemler, Peter ;
Temperton, Vicky M. ;
Hombach, Thomas ;
Streun, Matthias ;
Beer, Simone ;
Khodaverdi, Maryam ;
Ziemons, Karl ;
Coenen, Heinz H. ;
Schurr, Ulrich .
PLANT JOURNAL, 2009, 59 (04) :634-644
[9]   Visualizing three-dimensional root networks using computed tomography [J].
Kaestner, Anders ;
Schneebeli, Martin ;
Graf, Frank .
GEODERMA, 2006, 136 (1-2) :459-469
[10]   Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography [J].
Kaminuma, Eli ;
Yoshizumi, Takeshi ;
Wada, Takuji ;
Matsui, Minami ;
Toyoda, Tetsuro .
PLANT JOURNAL, 2008, 56 (03) :470-482