Nanocrystalline diamond:: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures

被引：101

作者：

Amaral, M. ^{[1
]}

Dias, A. G. ^{[2
,3
]}

Gomes, P. S. ^{[4
]}

Lopes, M. A. ^{[2
,3
]}

Silva, R. F. ^{[1
]}

Santos, J. D. ^{[2
,3
]}

Fernandes, M. H. ^{[4
]}

机构：

[1] Univ Aveiro, Dept Ceram & Glass Engn, CICECO, P-3810193 Aveiro, Portugal

[2] Univ Porto, Fac Engn, Seccao Mat DEMM, P-4200465 Oporto, Portugal

[3] Inst Biomed Engn, Lab Biomat, P-4150180 Oporto, Portugal

[4] Univ Porto, Fac Med Dent, Lab Farmacol & Biocompatibilidade Celular, P-4200393 Oporto, Portugal

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2008年 / 87A卷 / 01期

关键词：

cell culture; osteoblast; biocompatibility; diamond; joint replacement;

D O I：

10.1002/jbm.a.31742

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell Culture assays using MG63 osteoblast-like and human bone marrow, cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) Substrates for NCD coatings based on a previous demonstration Of the Superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue Culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. (C) 2007 Wiley Periodicals, Inc.

引用

页码：91 / 99

页数：9

共 47 条

[1] Enhanced performance of HFCVD nanocrystalline diamond self-mated tribosystems by plasma pretreatments on silicon nitride substrates [J].