Biotransformation of oleic acid into (E)-10-hydroxy-8-octadecenoic acid and (E)-7,10-dihydroxy-8-octadecenoic acid by Pseudomonas sp 42A2 in an immobilized system

被引：12

作者：

Culleré, J

Durany, O

Busquets, M

Manresa, A ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Microbiol Lab, E-08028 Barcelona, Spain

[2] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 03期

关键词：

biotransformation; hydroxy fatty acids; immobilisation; oleic acid; Pseudomonas;

D O I：

10.1023/A:1005679908262

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oleic acid was transformed into 10-hydroxy-8E-octadecenoic acid and 7,10-dihydroxy-8E-octadecenoic acid using Pseudomonas sp. 42A2 NCIMB 40045 in different immobilised supports. Celite R633 gave highest conversions in 48 h: the cellular yield (Y-p/x) g products g(-1) of cellular protein in the immobilised culture was 5 compared with the free-cell culture Y-p/x of 3.8. Conversion of oleic acid in the immobilised cell culture was 50% of the carbon source supplied.

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页码：215 / 219

页数：5

相关论文

共 9 条

[1] Biotransformation of oleic acid into 10-hydroxy-8E-octadecenoic acid by Pseudomonas sp 42A2 [J].

Bastida, J ;

de Andrés, C ;

Culleré, J ;

Busquets, M ;

Manresa, A .

BIOTECHNOLOGY LETTERS, 1999, 21 (12) :1031-1035

[2]

Blee E, 1995, INFORM, V6, P852

[3] 7,10-DIHYDROXY-8(E)-OCTADECENOIC ACID PRODUCED BY PSEUDOMONAS-42A2 - EVALUATION OF DIFFERENT CULTURAL PARAMETERS OF THE FERMENTATION [J].

DEANDRES, C ;

MERCADE, E ;

GUINEA, J ;

MANRESA, A .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1994, 10 (01) :106-109

[4] CONFINING MYCELIAL GROWTH TO POROUS MICROBEADS - A NOVEL TECHNIQUE TO ALTER THE MORPHOLOGY OF NON-NEWTONIAN MYCELIAL CULTURES [J].

GBEWONYO, K ;

WANG, DIC .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (04) :967-983

[5] Oxydation of oleic acid to (E)-10-hydroperoxy-8-octadecenoic and (E)-10-hydroxy-8-octadecenoic acids by Pseudomonas sp. 42A2 [J].

Guerrero, A ;

Casals, I ;

Busquets, M ;

Leon, Y ;

Manresa, A .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1997, 1347 (01) :75-81

[6] STRUCTURE AND SYNTHESIS OF UNSATURATED TRIHYDROXY C-18 FATTY-ACIDS IN RICE PLANT SUFFERING FROM RICE BLAST DISEASE [J].

KATO, T ;

YAMAGUCHI, Y ;

ABE, N ;

UYEHARA, T ;

NAMAI, T ;

KODAMA, M ;

SHIOBARA, Y .

TETRAHEDRON LETTERS, 1985, 26 (19) :2357-2360

[7] 4 FUNGITOXIC C-18 HYDROXY UNSATURATED FATTY-ACIDS FROM STROMATA OF EPICHLOE-TYPHINA [J].

KOSHINO, H ;

TOGIYA, S ;

YOSHIHARA, T ;

SAKAMURA, S ;

SHIMANUKI, T ;

SATO, T ;

TAJIMI, A .

TETRAHEDRON LETTERS, 1987, 28 (01) :73-76

[8] STEREOSELECTIVE SYNTHESIS OF UNSATURATED C-18 HYDROXY FATTY-ACIDS THE SELF DEFENSIVE SUBSTANCES [J].

RAO, AVR ;

REDDY, ER ;

PURANDARE, AV ;

VARAPRASAD, CVNS .

TETRAHEDRON, 1987, 43 (19) :4385-4394

[9] A study of a bacterial immobilization substratum for use in the bioremediation of crude oil in a saltwater system [J].

Wilson, NG ;

Bradley, G .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 83 (04) :524-530