Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders

被引：225

作者：

Monchaud, D. ^{[1
]}

Allain, C. ^{[1
]}

Bertrand, H. ^{[1
]}

Smargiasso, N. ^{[2
]}

Rosu, F. ^{[2
]}

Gabelica, V. ^{[2
]}

De Cian, A. ^{[3
,4
]}

Mergny, J. -L. ^{[3
,4
]}

Teulade-Fichou, M. -R ^{[1
]}

机构：

[1] Ctr Univ Paris XI, CNRS, Inst Curie, Sect Rech,UMR176, F-91405 Orsay, France

[2] Univ Liege, Inst Chim, Lab Spectrometrie Masse, GIGA R, B-4000 Liege, Belgium

[3] Museum Natl Hist Nat, Acides Nucl Dynam Ciblage & Fonct Biol, INSERM, U565,USM 503, F-75005 Paris, France

[4] Museum Natl Hist Nat, Lab Regulat & Dynam Genome, CNRS, UMR5153,USM 503, F-75005 Paris, France

来源：

BIOCHIMIE | 2008年 / 90卷 / 08期

关键词：

G-quadruplex DNA; thiazole orange; G-quadruplex ligands; fluorescent intercalator displacement;

D O I：

10.1016/j.biochi.2008.02.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the details of G4-FlD (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex-over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：1207 / 1223

页数：17

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