Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: Powerful analytical tools in recombinant protein chemistry

被引：95

作者：

Andersen, JS

Svensson, B

Roepstorff, P

机构：

[1] ODENSE UNIV, DEPT MOLEC BIOL, DK-5230 ODENSE M, DENMARK

[2] CARLSBERG LAB, DEPT CHEM, DK-2500 COPENHAGEN, DENMARK

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 04期

关键词：

D O I：

10.1038/nbt0496-449

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.

引用

页码：449 / 457

页数：9

共 112 条

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