Decoding protein modifications using top-down mass spectrometry

被引：375

作者：

Siuti, Nertila

Kelleher, Neil L.

机构：

[1] Univ Illinois, Dept Chem & Biochem, Urbana, IL 61801 USA

[2] Univ Illinois, Inst Genom Biol, Roger Adams Lab, Urbana, IL USA

来源：

NATURE METHODS | 2007年 / 4卷 / 10期

关键词：

D O I：

10.1038/NMETH1097

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.

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收藏

页码：817 / 821

页数：5

相关论文

共 49 条

[1] Whole protein dissociation in a quadrupole ion trap: Identification of an a priori unknown modified protein [J].

Amunugama, R ;

Hogan, JM ;

Newton, KA ;

McLuckey, SA .

ANALYTICAL CHEMISTRY, 2004, 76 (03) :720-727

[2] Proteomics by FTICR mass spectrometry: Top down and bottom up [J].

Bogdanov, B ;

Smith, RD .

MASS SPECTROMETRY REVIEWS, 2005, 24 (02) :168-200

[3] Precise characterization of human histories in the H2A gene family by top down mass spectrometry [J].

Boyne, MT ;

Pesavento, JJ ;

Mizzen, CA ;

Kelleher, NL .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (02) :248-253

[4] Identification of bacteriophage MS2 coat protein from E-coli lysates via ion trap collisional activation of intact protein ions [J].

Cargile, BJ ;

McLuckey, SA ;

Stephenson, JL .

ANALYTICAL CHEMISTRY, 2001, 73 (06) :1277-1285

[5] Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry [J].

Chi, An ;

Huttenhower, Curtis ;

Geer, Lewis Y. ;

Coon, Joshua J. ;

Syka, John E. P. ;

Bai, Dina L. ;

Shabanowitz, Jeffrey ;

Burke, Daniel J. ;

Troyanskaya, Olga G. ;

Hunt, Donald F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (07) :2193-2198

[6] Analysis of intact proteins on a chromatographic time scale by electron transfer dissociation tandem mass spectrometry [J].

Chi, An ;

Bai, Dina L. ;

Geer, Lewis Y. ;

Shabanowitz, Jeffrey ;

Hunt, Donald F. .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 259 (1-3) :197-203

[7] Chromatofocusing nonporous reversed-phase high-performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry of proteins from human breast cancer whole cell lysates: a novel two-dimensional liquid chromatography/mass spectrometry method [J].

Chong, BE ;

Yan, F ;

Lubman, DM ;

Miller, FR .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (04) :291-296

[8] Protein identification using sequential ion/ion reactions and tandem mass spectrometry [J].

Coon, JJ ;

Ueberheide, B ;

Syka, JEP ;

Dryhurst, DD ;

Ausio, J ;

Shabanowitz, J ;

Hunt, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9463-9468

[9] Pervasive combinatorial modification of histone H3 in human cells [J].

Garcia, Benjamin A. ;

Pesavento, James J. ;

Mizzen, Craig A. ;

Kelleher, Neil L. .

NATURE METHODS, 2007, 4 (06) :487-489

[10] Transit peptide cleavage sites of integral thylakoid membrane proteins [J].

Gómez, SM ;

Bil', KY ;

Aguilera, R ;

Nishio, JN ;

Faull, KF ;

Whitelegge, JP .

MOLECULAR & CELLULAR PROTEOMICS, 2003, 2 (10) :1068-1085

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