MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains

被引：58

作者：

Alonso, R ^{[1
]}

Mateo, E ^{[1
]}

Churruca, E ^{[1
]}

Martinez, I ^{[1
]}

Girbau, C ^{[1
]}

Fernández-Astorga, A ^{[1
]}

机构：

[1] Univ Basque Country, EHU, Fac Farm, Dept Inmunol Microbiol & Parasitol, Vitoria 01006, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2005年 / 63卷 / 01期

关键词：

Campylobacter; erythromycin; MAMA-PCR; mutation; resistance;

D O I：

10.1016/j.mimet.2005.03.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Synopsis. Twenty Campylobacter jejuni and 16 Campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. A combined mismatch amplification mutation assay-PCR technique was developed to detect the mutations A2074C and A2075G in the 23S rRNA gene associated with erythromycin resistance. All high-level erythromycin-resistant strains examined by DNA sequencing carried the transition mutation A2075G, whereas no isolate carried the A2074C mutation. No mutations were found among the susceptible and low-level erythromycin-resistant strains. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：99 / 103

页数：5

相关论文

共 10 条

[1]

Blaser MJ, 2000, MANDELL DOUGLAS BENN, P2276

[2] Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli [J].

Denis, M ;

Soumet, C ;

Rivoal, K ;

Ermel, G ;

Blivet, D ;

Salvat, G ;

Colin, P .

LETTERS IN APPLIED MICROBIOLOGY, 1999, 29 (06) :406-410

[3] Quinolone and macrolide resistance in Campylobacter jejuni and C-coli:: Resistance mechanisms and trends in human isolates [J].

Engberg, J ;

Aarestrup, FM ;

Taylor, DE ;

Gerner-Smidt, P ;

Nachamkin, I .

EMERGING INFECTIOUS DISEASES, 2001, 7 (01) :24-34

[4]

Friedman C.R., 2000, CAMPYLOBACTER, V2nd, P121

[5] Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in Berlin, Germany [J].

Luber, P ;

Wagner, J ;

Hahn, H ;

Bartelt, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (12) :3825-3830

[6]

National Committee for Clinical Laboratory Standards, 2002, M100S12 NCCLS

[7] Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni/coli by PCR and line probe assay [J].

Niwa, H ;

Chuma, T ;

Okamoto, K ;

Itoh, K .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2001, 18 (04) :359-364

[8] Relative contribution of target gene mutation and efflux to fluoroquinolone and erythromycin resistance, in French poultry and pig isolates of Campylobacter coli [J].

Payot, S ;

Avrain, L ;

Magras, C ;

Praud, K ;

Cloeckaert, A ;

Chaslus-Dancla, E .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2004, 23 (05) :468-472

[9] PCR-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in Campylobacter spp. [J].

Vacher, S ;

Ménard, A ;

Bernard, E ;

Mégraud, F .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (03) :1125-1128

[10] Ciprofloxacin resistance in Campylobacter jejuni isolates:: Detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis [J].

Zirnstein, G ;

Li, Y ;

Swaminathan, B ;

Angulo, F .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3276-3280