Hydrolysis of DNA by cerium(IV)/EDTA complex

被引：27

作者：

Kitamura, Y ^{[1
]}

Sumaoka, J ^{[1
]}

Komiyama, M ^{[1
]}

机构：

[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Meguro Ku, Tokyo 1538904, Japan

来源：

TETRAHEDRON | 2003年 / 59卷 / 52期

关键词：

cerium(IV); EDTA; DNA; hydrolysis; structure specificity;

D O I：

10.1016/j.tet.2003.06.005

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Homogeneous solutions are formed from equimolar amounts of Ce(NH4)(2)(NO3)(6) and EDTA. These solutions hydrolyze single-stranded DNA far more efficiently than double-stranded DNA. In contrast, Ce(IV) hydroxide gel, obtained in the absence of EDTA, hydrolyzes both single-stranded and double-stranded DNAs at almost the same rates. In order to achieve this substrate-specificity sufficiently, the mole ratio of EDTA to Ce(NH4)(2)(NO3)(6) must be 1.0 or greater. By using this remarkable specificity of Ce(IV)/EDTA complex, gap-site in substrate DNA is selectively hydrolyzed. The scission-site is modulated by use of appropriate combination of oligonucleotide additives. The reactions have been kinetically analyzed. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：10403 / 10408

页数：6

共 30 条

[1] Artificial dinuclear phosphoesterases [J].

Chin, J .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1997, 1 (04) :514-521

[2] Sequence-specific cleavage of RNA using macrocyclic lanthanide complexes conjugated to oligonucleotides: A structure activity study [J].

Hall, J ;

Husken, D ;

Haner, R .

NUCLEOSIDES & NUCLEOTIDES, 1997, 16 (7-9) :1357-1368

[3] Oligonucleotide conjugates of Eu(III) tetraazamacrocycles with pendent alcohol and amide groups promote sequence-specific RNA cleavage [J].

Huang, LY ;

Chappell, LL ;

Iranzo, O ;

Baker, BF ;

Morrow, JR .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2000, 5 (01) :85-92

[4] Hydrolysis of oligonucleotides by homogeneous Ce(IV)/EDTA complex [J].

Igawa, T ;

Sumaoka, J ;

Komiyama, M .

CHEMISTRY LETTERS, 2000, (04) :356-357

[5]

Kimura E, 1997, ADV INORG CHEM, V44, P229

[6] Preferential hydrolysis of gap and bulge sites in DNA by Ce(IV)/EDTA complex [J].

Kitamura, Y ;

Komiyama, M .

NUCLEIC ACIDS RESEARCH, 2002, 30 (19) :e102

[7] DNA HYDROLYSIS BY CERIUM(IV) DOES NOT INVOLVE EITHER MOLECULAR-OXYGEN OR HYDROGEN-PEROXIDE [J].

KOMIYAMA, M ;

SHIIBA, T ;

KODAMA, T ;

TAKEDA, N ;

SUMAOKA, J ;

YASHIRO, M .

CHEMISTRY LETTERS, 1994, (06) :1025-1028

[8] SEQUENCE-SPECIFIC AND HYDROLYTIC SCISSION OF DNA AND RNA BY LANTHANIDE COMPLEX-OLIGODNA HYBRIDS [J].

KOMIYAMA, M .

JOURNAL OF BIOCHEMISTRY, 1995, 118 (04) :665-670

[9] EFFICIENT AND OXYGEN-INDEPENDENT HYDROLYSIS OF SINGLE-STRANDED-DNA BY CERIUM(IV) ION [J].

KOMIYAMA, M ;

TAKEDA, N ;

TAKAHASHI, Y ;

UCHIDA, H ;

SHIIBA, T ;

KODAMA, T ;

YASHIRO, M .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1995, (02) :269-274

[10] CERIUM(IV)-OLIGODNA HYBRID AS HIGHLY SELECTIVE ARTIFICIAL NUCLEASE [J].

KOMIYAMA, M ;

SHIIBA, T ;

TAKAHASHI, Y ;

TAKEDA, N ;

MATSUMURA, K ;

KODAMA, T .

SUPRAMOLECULAR CHEMISTRY, 1994, 4 (01) :31-34

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