Virus-Poly(3,4-ethylenedioxythiophene) Composite Films for Impedance-Based Biosensing

被引：33

作者：

Donavan, Keith C. ^{[1
]}

Arter, Jessica A. ^{[1
]}

Pilolli, Rosa ^{[2
]}

Cioffi, Nicola ^{[2
]}

Weiss, Gregory A. ^{[1
]}

Penner, Reginald M. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[2] Univ Bari Aldo Moro, Dipartmento Chim, I-70126 Bari, Italy

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 07期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

COVALENT VIRUS LAYER; POLY(3,4-ETHYLENEDIOXYTHIOPHENE) PEDOT; PHAGE DISPLAY; ELECTRODES; AFFINITY; CELLS;

D O I：

10.1021/ac2000835

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Composite films composed of poly(3,4-ethylenedioxythiophene), PEDOT, and the filamentous virus M13-K07 were prepared by electrooxidation of 3,4-ethylenedioxythiophene (EDOT) in aqueous solutions containing 8 nM of the virus at planar gold electrodes. These films were characterized using atomic force microscopy and scanning electron microscopy. The electrochemical impedance of virus-PEDOT films increases upon exposure to an antibody (p-Ab) that selectively binds to the M13 coat peptide. Exposure to p-Ab causes a shift in both real (Z(RE)) and imaginary (Z(IM)) impedance components across a broad range of frequencies from 50 Hz to 10 kHz. Within a narrower frequency range from 250 Hz to 5 kHz, the increase of the total impedance (Z(total)) with p-Ab concentration conforms to a Langmuir adsorption isotherm over the concentration range from 6 to 66 nM, yielding a value for K-d = 16.9 nM at 1000 Hz.

引用

页码：2420 / 2424

页数：5

共 19 条

[1] Virus-PEDOT Nanowires for Biosensing [J].

Arter, Jessica A. ;

Taggart, David K. ;

McIntire, Theresa M. ;

Penner, Reginald M. ;

Weiss, Gregory A. .

NANO LETTERS, 2010, 10 (12) :4858-4862

[2] Toxicity evaluation of PEDOT/biomolecular composites intended for neural communication electrodes [J].

Asplund, M. ;

Thaning, E. ;

Lundberg, J. ;

Sandberg-Nordqvist, A. C. ;

Kostyszyn, B. ;

Inganas, O. ;

von Holst, H. .

BIOMEDICAL MATERIALS, 2009, 4 (04)

[3] Composite biomolecule/PEDOT materials for neural electrodes [J].

Asplund, Maria ;

von Holst, Hans ;

Inganas, Olle .

BIOINTERPHASES, 2008, 3 (03) :83-93

[4] Label-free impedance biosensors: Opportunities and challenges [J].

Daniels, Jonathan S. ;

Pourmand, Nader .

ELECTROANALYSIS, 2007, 19 (12) :1239-1257

[5] Impedance Biosensor for Peanut Protein Ara h 1 [J].

Huang, Yin ;

Bell, Melissa C. ;

Suni, Ian I. .

ANALYTICAL CHEMISTRY, 2008, 80 (23) :9157-9161

[6] Recent advances in electrochemical DNA hybridization sensors [J].

Hvastkovs, Eli G. ;

Buttry, Daniel A. .

ANALYST, 2010, 135 (08) :1817-1829

[7] Amperometric immunosensor for the detection of anti-West Nile Virus IgG [J].

Ionescu, Rodica E. ;

Cosnier, Serge ;

Herrmann, Sebastien ;

Marks, Robert S. .

ANALYTICAL CHEMISTRY, 2007, 79 (22) :8662-8668

[8] Poly(3,4-ethylenedioxythiophene) (PEDOT) nanobiointerfaces: Thin, ultrasmooth, and functionalized PEDOT films with in vitro and in vivo biocompatibility [J].

Luo, Shyh-Chyang ;

Ali, Emril Mohamed ;

Tansil, Natalia C. ;

Yu, Hsiao-Hua ;

Gao, Shujun ;

Kantchev, Eric A. B. ;

Ying, Jackie Y. .

LANGMUIR, 2008, 24 (15) :8071-8077

[9] Virus-Based Chemical and Biological Sensing [J].

Mao, Chuanbin ;

Liu, Aihua ;

Cao, Binrui .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (37) :6790-6810

[10] Highly sensitive phage-based biosensor for the detection of β-galactosidase [J].

Nanduri, Viswaprakash ;

Balasubramanian, Shankar ;

Sista, Srinivas ;

Vodyanoy, Vitaly J. ;

Simonian, Aleksandr L. .

ANALYTICA CHIMICA ACTA, 2007, 589 (02) :166-172

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