Subnanomolar cyanide detection at polyphenol oxidase/clay biosensors

被引：305

作者：

Shan, D ^{[1
]}

Mousty, C ^{[1
]}

Cosnier, S ^{[1
]}

机构：

[1] Univ Grenoble 1, Lab Electrochim Organ & Photochim Redox, UMR CNRS 5630, Inst Chim Mol Grenoble,FR CNRS 2607, Grenoble, France

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 01期

关键词：

D O I：

10.1021/ac034713m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel, inexpensive, and simple amperometric biosensor based on immobilization of polyphenol oxidase (PPO) into Zn-Al layered double hydroxides, also called anionic clays, is applied for determination of cyanide. The detection of cyanide was performed via its inhibiting action on the PPO electrode. Measurement was carried out with 3,4-dihydroxyphenylacetic acid as enzyme substrate, the enzymatically generated quinoid products being electro-reduced at -0.2 V. An extremely sensitive detection limit (0.1 nM) was obtained for cyanide. Enzyme immobilization into an anionic exchanger clay seems to cause an increase in cyanide inhibition effects because of anion accumulation in the clay matrix.

引用

页码：178 / 183

页数：6

共 33 条

[21]

Nollet L.M.L., 2000, HDB WATER ANAL, V102, P367

[22] Development of a sol-gel enzyme inhibition-based amperometric biosensor for cyanide [J].

Park, TM ;

Iwuoha, EI ;

Smyth, MR .

ELECTROANALYSIS, 1997, 9 (14) :1120-1123

[23]

RAWN JD, 1990, TRAITE BIOCH, P176

[24] Peroxidase-modified electrodes: Fundamentals and application [J].

Ruzgas, T ;

Csoregi, E ;

Emneus, J ;

Gorton, L ;

MarkoVarga, G .

ANALYTICA CHIMICA ACTA, 1996, 330 (2-3) :123-138

[25] Layered double hydroxides: An attractive material for electrochemical biosensor design [J].

Shan, D ;

Cosnier, S ;

Mousty, C .

ANALYTICAL CHEMISTRY, 2003, 75 (15) :3872-3879

[26] HRP wiring by redox active layered double hydroxides:: Application to the mediated H2O2 detection [J].

Shan, D ;

Cosnier, S ;

Mousty, C .

ANALYTICAL LETTERS, 2003, 36 (05) :909-922

[27] CYANIDE DETECTION USING A SUBSTRATE-REGENERATING, PEROXIDASE-BASED BIOSENSOR [J].

SMIT, MH ;

CASS, AEG .

ANALYTICAL CHEMISTRY, 1990, 62 (22) :2429-2436

[28] TOXIN DETECTION USING A TYROSINASE-COUPLED OXYGEN-ELECTRODE [J].

SMIT, MH ;

RECHNITZ, GA .

ANALYTICAL CHEMISTRY, 1993, 65 (04) :380-385

[29] H2O2-generating peroxidase electrodes as reagentless cyanide sensors [J].

Tatsuma, T ;

Oyama, N .

ANALYTICAL CHEMISTRY, 1996, 68 (09) :1612-1615

[30] Cyanide determination by an ISFET-based peroxidase biosensor [J].

Volotovsky, V ;

Kim, N .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (09) :1029-1033

← 1 2 3 4 →