Amplified electrochemiluminescence of quantum dots by electrochemically reduced graphene oxide for nanobiosensing of acetylcholine

被引：81

作者：

Deng, Shengyuan ^{[1
]}

Lei, Jianping ^{[1
]}

Cheng, Lingxiao ^{[1
]}

Zhang, Yangyang ^{[1
]}

Ju, Huangxian ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Graphene; Electrochemical reduction; Electrochemiluminescence; Quantum dots; Biosensing; Acetylcholine; LARGE-AREA; BIOSENSOR; FUNCTIONALIZATION; REDUCTION; SHEETS; FILM;

D O I：

10.1016/j.bios.2011.05.023

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A signal amplification system for electrochemiluminescence (ECL) of quantum dots (QDs) was developed by using electrochemically reduced graphene oxide (ERGO) to construct a nanobiosensing platform. Due to the structural defects of GO, the ECL emission of QDs coated on GO modified electrode was significantly quenched. After the electrochemical reduction of GO, the restoration of structural conjugation was observed with spectroscopic, morphologic and impedance techniques. Thus in the presence of dissolved 02 as coreactant, the QDs/ERGO modified electrode showed ECL intensity increase by 4.2 and 178.9 times as compared with intrinsic QDs and QDs/GO modified electrodes due to the adsorption of dissolved O-2 on ERGO and the facilitated electron transfer. After choline oxidase (ChO) or ChO-acetylcholinesterase was further covalently cross-linked on the QDs/ERGO modified electrode, two ECL biosensors for choline and acetylcholine were fabricated, which showed the linear response ranges and detection limits of 10-210 mu M and 8.8 mu M for choline, and 10-250 mu M and 4.7 mu M for acetylcholine, respectively. This green and facile approach to prepare graphene-QDs system could be of potential applications in electronic device and bioanalysis. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：4552 / 4558

页数：7

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