Anti-angiogenic activity and phytochemical screening of fruit fractions from Vitex agnus castus

被引：32

作者：

Certo, Giovanna ^{[1
]}

Costa, Rosaria ^{[2
]}

D'Angelo, Valeria ^{[3
]}

Russo, Marina ^{[4
]}

Albergamo, Ambrogina ^{[2
,5
]}

Dugo, Giacomo ^{[5
]}

Germano, Maria Paola ^{[3
]}

机构：

[1] Univ Messina, Fdn Prof Antonio Imbesi, Messina, Italy

[2] Univ Messina, Dept Biomed Dent Morphol & Funct Images Sci, Messina, Italy

[3] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy

[4] Univ Campus Biomed, CIR, Rome, Italy

[5] Univ Messina, Science4life Srl, Messina, Italy

来源：

NATURAL PRODUCT RESEARCH | 2017年 / 31卷 / 24期

关键词：

Chasteberry; angiogenesis; zebrafish; chick chorioallantoic membrane; RP-HPLC-PDA; RP-HPLC-MS; L; PLANTS; ASSAYS; JUICE; OIL;

D O I：

10.1080/14786419.2017.1303696

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Although the antitumour activity of Vitex agnus castus fruits has been already addressed, no work has yet assessed their anti-angiogenic potential. To this purpose, several extractive fractions of such fruits were tested on zebrafish embrios by EAP assay, so that only the bioactive fractions could be subsequently tested on the chick chorioallantoic membrane by CAM assay. Bioactive fractions were also phytochemically screened to identify those bioactive compounds responsible for anti-angiogenic activity. A marked inhibition of vessel formation was detected only in zebrafish embryos treated with chloroform or ethyl acetate fractions. Considering CAM assay, chloroform fraction induced a strong reduction of microvasculature and haemoglobin content; while lower anti-angiogenic effects of the ethyl acetate fraction were determined. Phytochemical analyses confirmed the presence of several bioactive anti-angiogenic compounds. Overall, obtained preliminary results highlighted a potential anti-angiogenic activity of V. agnus castus fruits. [GRAPHICS]

引用

页码：2850 / 2856

页数：7

共 26 条

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