Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant

被引：64

作者：

Sekiya, H

Mima, T

Morita, Y

Kuroda, T

Mizushima, T

Tsuchiya, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Dept Microbiol, Okayama 7008530, Japan

[2] Okayama Univ, Ctr Gene Res, Okayama 7008530, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 09期

关键词：

D O I：

10.1128/AAC.47.9.2990-2992.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.

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页码：2990 / 2992

页数：3

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