Ion mobility-mass spectrometry analysis of large protein complexes

被引：915

作者：

Ruotolo, Brandon T. ^{[1
]}

Benesch, Justin L. P. ^{[1
]}

Sandercock, Alan M. ^{[1
]}

Hyung, Suk-Joon ^{[1
]}

Robinson, Carol V. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 07期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/nprot.2008.78

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a detailed protocol for both data collection and interpretation with respect to ion mobility-mass spectrometry analysis of large protein assemblies. Ion mobility is a technique that can separate gaseous ions based on their size and shape. Specifically, within this protocol, we cover general approaches to data interpretation, methods of predicting whether specific model structures for a given protein assembly can be separated by ion mobility, and generalized strategies for data normalization and modeling. The protocol also covers basic instrument settings and best practices for both observation and detection of large noncovalent protein complexes by ion mobility-mass spectrometry.

引用

页码：1139 / 1152

页数：14

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