Shotgun cloning and heterologous expression of the patellamide gene cluster as a strategy to achieving sustained metabolite production

被引：129

作者：

Long, PF

Dunlap, WC

Battershill, CN

Jaspars, M

机构：

[1] Univ Aberdeen, Dept Chem, Aberdeen AB24 3UE, Scotland

[2] Univ London, Sch Pharm, London WC1N 1AX, England

[3] Australian Inst Marine Sci, Marine Biotechnol Grp, Townsville, Qld 4810, Australia

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 10期

关键词：

peptides; Escherichia coli; heterologous expression; HPLC-MS; natural products; patellamides;

D O I：

10.1002/cbic.200500210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Shotgun cloning into E. coli of genomic DNA from Prochloron sp., symbiont of the seasquirt Lissoclinum patella, resulted in the heterologous expression of the patellamide gene cluster and subsequent production of patellamide D (1) and ascidiacyclamide (2) at levels of 80-100 ng mL-1. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1760 / 1765

页数：6

共 38 条

[1] Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage
Béjà, O
Suzuki, MT
Koonin, EV
Aravind, L
Hadd, A
Nguyen, LP
Villacorta, R
Amjadi, M
Garrigues, C
Jovanovich, SB
Feldman, RA
DeLong, EF
[J]. ENVIRONMENTAL MICROBIOLOGY, 2000, 2 (05) : 516 - 529
[2] Precursor-directed biosynthesis of epothilone in Escherichia coli
Boddy, CN
Hotta, K
Tse, ML
Watts, RE
Khosla, C
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (24) : 7436 - 7437
[3] The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases
Cane, DE
Walsh, CT
[J]. CHEMISTRY & BIOLOGY, 1999, 6 (12): : R319 - R325
[4] The barbamide biosynthetic gene cluster: a novel marine cyanobacterial system of mixed polyketide synthase (PKS)-non-ribosomal peptide synthetase (NRPS) origin involving an unusual trichloroleucyl starter unit
Chang, ZX
Flatt, P
Gerwick, WH
Nguyen, VA
Willis, CL
Sherman, DH
[J]. GENE, 2002, 296 (1-2) : 235 - 247
[5] Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)
Chong, PP
Podmore, SM
Kieser, HM
Redenbach, M
Turgay, K
Marahiel, M
Hopwood, DA
Smith, CP
[J]. MICROBIOLOGY-UK, 1998, 144 : 193 - 199
[6] ASCIDIANS - PRODUCERS OF AMINO-ACID DERIVED METABOLITES
DAVIDSON, BS
[J]. CHEMICAL REVIEWS, 1993, 93 (05) : 1771 - 1791
[7] Evidence for the biosynthesis of bryostatins by the bacterial symbiont "Candidatus Endobugula sertula" of the bryozoan Bugula neritina
Davidson, SK
Allen, SW
Lim, GE
Anderson, CM
Haygood, MG
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (10) : 4531 - 4537
[8] NEW CYCLIC-PEPTIDES WITH CYTO-TOXIC ACTIVITY FROM THE ASCIDIAN LISSOCLINUM-PATELLA
DEGNAN, BM
HAWKINS, CJ
LAVIN, MF
MCCAFFREY, EJ
PARRY, DL
VANDENBRENK, AL
WATTERS, DJ
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1989, 32 (06) : 1349 - 1354
[9] Microbial technologies for the discovery of novel bioactive metabolites
Donadio, S
Monciardini, P
Alduina, R
Mazza, P
Chiocchini, C
Cavaletti, L
Sosio, M
Puglia, AM
[J]. JOURNAL OF BIOTECHNOLOGY, 2002, 99 (03) : 187 - 198
[10] Lyngbyatoxin biosynthesis: Sequence of biosynthetic gene cluster and identification of a novel aromatic prenyltransferase
Edwards, DJ
Gerwick, WH
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (37) : 11432 - 11433

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