Surface plasmon resonance biosensors as a tool in antibody engineering

被引：35

作者：

Alfthan, K ^{[1
]}

机构：

[1] VTT Biotechnol & Food Res, FIN-02044 Espoo, VTT, Finland

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 06期

关键词：

surface plasmon resonance; real-time interaction analysis; biosensor; antibody engineering; phage display; BIAcore;

D O I：

10.1016/S0956-5663(98)00020-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Modern gene technology combined with efficient microbial expression systems provides tools to produce antibodies with reduced functional size and improved binding properties as well as antibody fusions or novel antibodies. Surface plasmon resonance based biosensors, which measure antigen-antibody interactions in real-time, can be used for a diverse characterization of the modified antibodies. To date, the majority of published work originates from real-time biospecific interaction analysis based on the BIAcore(R) instruments. This article describes the range of applications in antibody engineering in which BIAcore has been applied. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：653 / 663

页数：11

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