Simultaneous determination of carbamate and organophosphorus pesticides in honeybees by liquid chromatography-mass spectrometry

被引：13

作者：

Fernández, M ^{[1
]}

Picó, Y ^{[1
]}

Mañes, J ^{[1
]}

机构：

[1] Univ Valencia, Fac Farm, Lab Bromatol & Toxicol, E-46100 Burjassot, Valencia, Spain

来源：

CHROMATOGRAPHIA | 2003年 / 58卷 / 3-4期

关键词：

column liquid chromatography - mass spectrometry; atmospheric pressure chemical ionization and electrospray ionization; carbamate and organophosphorus pesticides; honeybees;

D O I：

10.1365/s10337-003-0022-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential of liquid chromatography-mass spectrometry (LC-MS) has been studied for the simultaneous determination of sixteen carbamate and organophosphorus pesticides in honeybees using a traditional sample preparation protocol based on acetone extraction and dichloromethane partitioning. The performances of both atmospheric pressure chemical ionization (APCl) and electrosproy (ES) interfaces were compared. APCl offered better sensitivity and specificity for a higher range of pesticides. Limits of quantification were from 0.01 to 0.17 mg kg(-1), at which recoveries obtained were between 64 and 93%, except for pirimicarb that was at 13%, with relative standard deviations ranging from 7 to 20%. Fenitrothion, fenoxycarb, methiocarb and phoxim were found in bees from Valencian Community beehives at concentrations between 0.03 and 3.75 mg kg(-1).

引用

页码：151 / 158

页数：8

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