Liquid phase immunoassay using magnetic markers and superconducting quantum interference device

被引：16

作者：

Enpuku, Keiji ^{[1
]}

Tanaka, Tsuyoshi

Matsuda, Takashi

Kuma, Hiroyuki

Hamasaki, Naotaka

Dang, Feng

Enomoto, Naoya

Hojo, Junichi

Yoshinaga, Kohji

Ludwig, Frank

Ghaffari, Fatemeh

Heim, Erik

Schilling, Meinhard

机构：

[1] Kyushu Univ, Res Inst Supercond Sci & Syst, Fukuoka 8190395, Japan

[2] Nagasaki Int Univ, Fac Pharmaceut Sci, Nagasaki 8593298, Japan

[3] Kyushu Univ, Dept Appl Chem, Fukuoka 8190395, Japan

[4] Kyushu Inst Technol, Dept Appl Chem, Kitakyushu, Fukuoka 8048550, Japan

[5] Tech Univ Carolo Wilhelmina Braunschweig, Inst Elect Measurement & Fund Elect Engn, D-38106 Braunschweig, Germany

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS | 2007年 / 46卷 / 11期

关键词：

liquid phase immunoassay; biosensor; SQUID; magnetic marker; Brownian rotation;

D O I：

10.1143/JJAP.46.7524

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

A liquid phase immunoassay utilizing magnetic markers and a high-T-c superconducting quantum interference device (SQUID) was studied. In this method, the biological target is detected using magnetic markers, i.e., the magnetic signal from the markers that bound to the target is detected with the SQUID. The detection was performed in a solution containing both the bound and unbound (free) markers without using the so-called bound/free (BF) separation process. The bound markers were distinguished from the free markers by utilizing the Brownian rotation of the free markers. First, the properties of the free markers in the solution, such as the M-H curve and magnetic relaxation, were measured to study the background signal from the free markers. Markers that exhibit remanence were used for the experiment. Using the obtained results, we discuss the effects of the residual earth field and aggregation of the markers on the background signal. Next, we detected a fungus, Candida albicans, with the described liquid phase immunoassay. Good relationship was obtained between the detected signal and the number of fungi. The minimum detectable number of fungi was as small as 30.

引用

页码：7524 / 7529

页数：6

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