Rapid detection of S-adenosyl homocysteine using self-assembled optical diffraction gratings

被引：27

作者：

Acharya, Ghanashyam ^{[3
]}

Chang, Chun-Li ^{[3
]}

Holland, David P. ^{[1
]}

Thompson, David H. ^{[1
]}

Savran, Cagri A. ^{[2
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Weldon Sch Biomed Engn, Sch Mech Engn, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA

[3] Purdue Univ, Sch Elect & Comp Engn, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 06期

关键词：

aptamers; biosensors; diffraction; S-adenosyl homocysteine; self-assembly;

D O I：

10.1002/anie.200703222

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) I saw the SAH: Clinically relevant concentrations of S-adenosyl homocysteine (SAH) can be rapidly detected using self-assembled diffraction gratings. SAH bound to antibody-conjugated (Ab) microbeads (see picture) and then incubated with a gold chip functionalized with SAH-specific aptamer in alternating line patterns produces gratings that are detected by optical diffraction intensity measurements (detection limit: 64 pmol L -1). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1051 / 1053

页数：3

共 26 条

[1] Real-time multicolor DNA detection with chemoresponsive diffraction gratings and nanoparticle probes [J].

Bailey, RC ;

Nam, JM ;

Mirkin, CA ;

Hupp, JT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (44) :13541-13547

[2] Determination of protein content using a solid phase spectrophotometric procedure [J].

Capitán-Vallvey, LF ;

Duque, O ;

Mirón-García, G ;

Checa-Moreno, R .

ANALYTICA CHIMICA ACTA, 2001, 433 (01) :155-163

[3]

CHANG CL, 2007, APPL PHYS LETT, V90

[4] Preparation of novel composite nanoclusters and their application in the ultrasensitive detection of proteins [J].

Chen, HQ ;

Wang, L ;

Wang, LY ;

Wang, GF ;

Li, L ;

Xu, FG .

ANALYTICA CHIMICA ACTA, 2004, 521 (01) :9-15

[5] Electrocatalytic detection of aurothiomalate on carbon electrodes -: Application as a non-enzymatic label to the quantification of proteins [J].

de la Escosura-Muñiz, A ;

González-García, MB ;

Costa-García, A .

ANALYTICA CHIMICA ACTA, 2004, 524 (1-2) :355-363

[6] Development of an immunosensor for the determination of rabbit IgG using streptavidin modified screen-printed carbon electrodes [J].

Díaz-González, M ;

Hernández-Santos, D ;

González-García, MB ;

Costa-García, A .

TALANTA, 2005, 65 (02) :565-573

[7] Implantable chemical sensors for real-time clinical monitoring: progress and challenges [J].

Frost, MC ;

Meyerhoff, ME .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2002, 6 (05) :633-641

[8] RNA aptamers to S-adenosylhomocysteine:: Kinetic properties, divalent cation dependency, and comparison with anti-S-adenosylhomocysteine antibody [J].

Gebhardt, K ;

Shokraei, A ;

Babaie, E ;

Lindqvist, BH .

BIOCHEMISTRY, 2000, 39 (24) :7255-7265

[9] Stable-isotope dilution liquid chromatography-electrospray injection tandem mass spectrometry method for fast, selective measurement of S-adenosylmethionine and S-adenosylhomocysteine in plasma [J].

Gellekink, H ;

Van Oppenraaij-Emmerzaal, D ;

van Rooij, A ;

Struys, EA ;

den Heijer, M ;

Blom, HJ .

CLINICAL CHEMISTRY, 2005, 51 (08) :1487-1492

[10] Application of the dibromohydroxyphenylfluorone-molybdenum(VI) complex to the sensitive spectrophotometric determination of protein [J].

Guo, ZX ;

Hao, YM ;

Cong, X ;

Shen, HX .

ANALYTICA CHIMICA ACTA, 2000, 403 (1-2) :225-233

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