Room temperature ionic liquid doped DNA network immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide

被引：65

作者：

Guo, Cunlan ^{[1
]}

Song, Yonghai ^{[1
]}

Wei, Hui ^{[1
]}

Li, Peicai ^{[1
]}

Wang, Li ^{[1
]}

Sun, Lanlan ^{[1
]}

Sun, Yujing ^{[1
]}

Li, Zhuang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 02期

基金：

中国国家自然科学基金;

关键词：

DNA; room temperature ionic liquid; horseradish peroxidase; hydrogen peroxide; biosensor;

D O I：

10.1007/s00216-007-1478-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel electrochemical H2O2 biosensor was constructed by embedding horseradish peroxide (HRP) in a 1-butyl-3-methylimidazolium tetrafluoroborate doped DNA network casting on a gold electrode. The HRP entrapped in the composite system displayed good electrocatalytic response to the reduction of H2O2. The composite system could provide both a biocompatible microenvironment for enzymes to keep their good bioactivity and an effective pathway of electron transfer between the redox center of enzymes, H2O2 and the electrode surface. Voltammetric and time-based amperometric techniques were applied to characterize the properties of the biosensor. The effects of pH and potential on the amperometric response to H2O2 were studied. The biosensor can achieve 95% of the steady-state current within 2 s response to H2O2. The detection limit of the biosensor was 3.5 mu M, and linear range was from 0.01 to 7.4 mM. Moreover, the biosensor exhibited good sensitivity and stability. The film can also be readily used as an immobilization matrix to entrap other enzymes to prepare other similar biosensors.

引用

页码：527 / 532

页数：6

共 35 条

[1] Ionic liquids in analytical chemistry [J].