Low-molecular-weight spies of protein-protein interactions

被引：3

作者：

Blobel, Jascha ^{[1
]}

Fayos, Rosa ^{[1
]}

Garcia, Jesus ^{[1
]}

Marimon, Oriol ^{[1
,2
]}

Perez, Yolanda ^{[1
]}

Pons, Miquel ^{[1
,2
]}

机构：

[1] Inst Res Biomed, Glassboro, NJ 08028 USA

[2] Univ Barcelona, Dept Organ Chem, E-08028 Barcelona, Spain

来源：

COMPTES RENDUS CHIMIE | 2008年 / 11卷 / 4-5期

关键词：

protein-ligand interactions; protein-protein interactions by STD; molecular spies; NMR;

D O I：

10.1016/j.crci.2007.08.015

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The interaction of low-molecular-weight ligands with proteins in the search for new selective drugs is driving great efforts in the pharmaceutical industry and academic research. The same principles can be applied to solve an equally challenging task: selective monitoring of protein interactions by NMR at concentrations close to physiological ones in unlabelled samples. In this review, we discuss different approaches to this problem through the measurement of either relaxation rates or chemical shifts of free ligand spies in equilibrium with their macromolecular targets.

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收藏

页码：499 / 505

页数：7

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