Immobilization of Streptomyces olivaceoviridis E-86 xylanase on Eudragit S-100 for xylo-oligosaccharide production

被引：85

作者：

Ai, ZL ^{[1
]}

Jiang, ZQ ^{[1
]}

Li, LT ^{[1
]}

Deng, W ^{[1
]}

Kusakabe, I ^{[1
]}

Li, HS ^{[1
]}

机构：

[1] China Agr Univ, Dept Biotechnol, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 08期

关键词：

Eudragit S-100; immobilization; Streptomyces olivaceoviridis; xylanase; xylo-oligosaccharide;

D O I：

10.1016/j.procbio.2004.12.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 47 kDa xylanase (FXYN) from Streptomyces olivaceoviridis E-86 was immobilized on Eudragit S-100 and an immobilization efficiency of 90% was obtained. The optimum pH of the immobilized xylanase shifted from 5.8 to 6.3 while its optimum temperature moved from 60 to 65 degrees C. The immobilized enzyme also showed a higher thermal stability than the free xylanase. Only a marginal increase in the K-m values of free enzyme was observed upon immobilization. However, V-max values of the immobilized enzyme were 82-161% higher than those of the free xylanase. The immobilized xylanase was also evaluated for its application in hydrolyzing the corncob powder pre-treated with 2% NaOH solution for xylo-oligosaccharide production. The final extent of xylan hydrolysis using pre-treated corncob powder was 84% for the immobilized enzyme after 24 h of incubation at 55 degrees C. The immobilized xylanase retained 81% of its initial hydrolysis activity even after being recycled four times. Therefore, the immobilized xylanase is suitable to produce xylo-oligosaccahrides from corncob powder pre-treated with dilute alkaline solution. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2707 / 2714

页数：8

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