Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease

被引：19

作者：

Abbiss, Hayley ^{[1
,3
,4
]}

Maker, Garth L. ^{[4
,5
]}

Gummer, Joel ^{[3
]}

Sharman, Matthew J. ^{[2
]}

Phillips, Jacqueline K. ^{[6
]}

Boyce, Mary ^{[1
]}

Trengove, Robert D. ^{[3
,5
]}

机构：

[1] Edith Cowan Univ, Sch Nat Sci, Perth, WA, Australia

[2] Edith Cowan Univ, Sch Exercise & Hlth Sci, Perth, WA, Australia

[3] Murdoch Univ, Separat Sci & Metabol Lab, Perth, WA, Australia

[4] Murdoch Univ, Sch Vet & Biomed Sci, Perth, WA, Australia

[5] Murdoch Univ Node, Metabol Australia, Perth, WA, Australia

[6] Macquarie Univ, Australian Sch Adv Med, Sydney, NSW 2109, Australia

来源：

NEPHROLOGY | 2012年 / 17卷 / 02期

关键词：

biomarker; gas chromatography; metabolite; polycystic kidney disease; urine; BIOLOGICAL SAMPLES; EXPRESSION; CHALLENGES;

D O I：

10.1111/j.1440-1797.2011.01532.x

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Aim: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. Methods: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. Results: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. Conclusion: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.

引用

页码：104 / 110

页数：7

共 34 条

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