Long-term serological follow up and cross-challenge studies in rhesus monkeys experimentally infected with hepatitis E virus

被引:33
作者
Arankalle, VA [1 ]
Chadha, MS [1 ]
Chobe, LP [1 ]
机构
[1] Natl Inst Virol, Hepatitis Div, Pune 411001, Maharashtra, India
关键词
cross-challenge; hepatitis E virus; IgG-anti-HEV; titers; long-term follow up; rhesus monkeys;
D O I
10.1016/S0168-8278(99)80062-2
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: The aims of this study were to examine the decline of IgG anti-HEV antibodies over a period of 7 years in rhesus monkeys experimentally infected with hepatitis E virus, and to assess the protectivity of these antibodies by challenging the monkeys with a heterologous isolate of hepatitis E virus, 5 years after the primary inoculation. Methods: Nine rhesus monkeys (six non-pregnant and three pregnant at the time of hepatitis E virus inoculation) were followed serologically and biochemically for 7 years post-inoculation. Based on regression analysis, estimated time for IgG anti-HEV titers to reach 1:100 or 1:50 was calculated, Three of the monkeys inoculated initially with AKL-90 isolate and challenged 2 years later with PUN-85 isolate of hepatitis E virus were rechallenged with KOL-91 isolate of the virus, 5 years post-primary inoculation. Evidence of viral replication was assessed by measuring serum alanine aminotransferase levels, excretion of the virus in feces or bile (reverse-transcription polymerase chain reaction) and rise in IgG anti-HEV titers (ELISA), Results: None of the challenged monkeys showed evidence of disease. In contrast to extensive replication of the virus in anti-HEV-negative control monkeys, limited replication was noted in one of the challenged monkeys. The estimated time for the titers to reach 1:100 or 1:50 varied from 3.15 to 44.9 years (19.4+/-11.6 years) and 6.9 to 84.3 years (35.4+/-21.3 years), respectively, Decline in titers was independent of the pregnancy status at the time of infection or reexposure of the monkeys to HEV; Conclusion: The results show persistence of IgG anti-HEV antibodies for a long time and protectivity of low titered antibodies against reinfection, leading to disease even after intravenous exposure to a heterologous isolate of hepatitis E virus.
引用
收藏
页码:199 / 204
页数:6
相关论文
共 20 条
[11]   SEROLOGIC IDENTIFICATION OF HEPATITIS-E VIRUS-INFECTIONS IN EPIDEMIC AND ENDEMIC SETTINGS [J].
FAVOROV, MO ;
FIELDS, HA ;
PURDY, MA ;
YASHINA, TL ;
ALEKSANDROV, AG ;
ALTER, MJ ;
YARASHEVA, DM ;
BRADLEY, DW ;
MARGOLIS, HS .
JOURNAL OF MEDICAL VIROLOGY, 1992, 36 (04) :246-250
[12]   ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DIAGNOSIS OF ACUTE SPORADIC HEPATITIS-E IN EGYPTIAN CHILDREN [J].
GOLDSMITH, R ;
YARBOUGH, PO ;
REYES, GR ;
FRY, KE ;
GABOR, KA ;
KAMEL, M ;
ZAKARIA, S ;
AMER, S ;
GAFFAR, Y .
LANCET, 1992, 339 (8789) :328-331
[13]  
GUPTA H, 1990, INDIAN J MED RES-A, V91, P87
[14]  
KHUROO MS, 1993, LANCET, V341, P1355
[15]   INCIDENCE AND SEVERITY OF VIRAL-HEPATITIS IN PREGNANCY [J].
KHUROO, MS ;
TELI, MR ;
SKIDMORE, S ;
SOFI, MA ;
KHUROO, MI .
AMERICAN JOURNAL OF MEDICINE, 1981, 70 (02) :252-255
[16]  
PILLOT J, 1995, CR ACAD SCI III-VIE, V318, P1059
[17]   HEPATITIS-E VIRUS - THE CAUSE OF A WATERBORNE HEPATITIS OUTBREAK [J].
SKIDMORE, SJ ;
YARBOUGH, PO ;
GABOR, KA ;
REYES, GR .
JOURNAL OF MEDICAL VIROLOGY, 1992, 37 (01) :58-60
[18]   SUCCESSFUL PASSIVE AND ACTIVE IMMUNIZATION OF CYNOMOLGUS MONKEYS AGAINST HEPATITIS-E [J].
TSAREV, SA ;
TSAREVA, TS ;
EMERSON, SU ;
GOVINDARAJAN, S ;
SHAPIRO, M ;
GERIN, JL ;
PURCELL, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10198-10202
[19]   Recombinant vaccine against hepatitis E: dose response and protection against heterologous challenge [J].
Tsarev, SA ;
Tsareva, TS ;
Emerson, SU ;
Govindarajan, S ;
Shapiro, M ;
Gerin, JL ;
Purcell, RH .
VACCINE, 1997, 15 (17-18) :1834-1838
[20]   ELISA FOR ANTIBODY TO HEPATITIS-E VIRUS (HEV) BASED ON COMPLETE OPEN-READING FRAME-2 PROTEIN EXPRESSED IN INSECT CELLS - IDENTIFICATION OF HEV INFECTION IN PRIMATES [J].
TSAREV, SA ;
TSAREVA, TS ;
EMERSON, SU ;
KAPIKIAN, AZ ;
TICEHURST, J ;
LONDON, W ;
PURCELL, RH .
JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (02) :369-378