Production of tannase by Aspergillus niger Aa-20 in submerged and solid-state fermentation:: influence of glucose and tannic acid

被引:105
作者
Aguilar, CN
Augur, C
Favela-Torres, E
Viniegra-González, G
机构
[1] Univ Autonoma Metropolitana, Unidad Iztapalapa, Dept Biotechnol, Mexico City 09340, DF, Mexico
[2] IRD, Paris, France
[3] Univ Autonoma Coahuila, Food Res Dept, Saltillo, Coahuila, Mexico
关键词
tannase; glucose; tannic acid; submerged and solid-state fermentation;
D O I
10.1038/sj.jim.7000132
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Tannase production by Aspergillus niger Aa-20 was studied in submerged (SmF) and solid-state (SSF) fermentation systems with different tannic acid and glucose concentrations. Tannase activity and productivity were at least 2.5 times higher in SSF than in SmF. Addition of high tannic acid concentrations increased total tannase activity in SSF, while in SmF it was decreased. In SmF, total tannase activity increased from 0.57 to 1.03 IU/mL, when the initial glucose concentration increased from 6.25 to 25 g/L, but a strong catabolite repression of tannase synthesis was observed in SmF when an initial glucose concentration of 50 g/L was used. In SSF, maximal values of total tannase activity decreased from 7.79 to 2.51 IU when the initial glucose concentration was increased from 6.25 to 200 g/L. Kinetic results on tannase production indicate that low tannase activity titers in SmF could be associated to an enzyme degradation process which is not present in SSF. Tannase titers produced by A. niger Aa-20 are fermentation system-dependent, favoring SSF over SmF.
引用
收藏
页码:296 / 302
页数:7
相关论文
共 27 条
[1]  
AGUILAR CN, 2001, P BIOCH, P565
[2]   PURIFICATION AND SOME PROPERTIES OF YEAST TANNASE [J].
AOKI, K ;
SHINKE, R ;
NISHIRA, H .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (01) :79-85
[3]   INFLUENCE OF MOLD GROWTH ON THE PRESSURE-DROP IN AERATED SOLID-STATE FERMENTERS [J].
AURIA, R ;
MORALES, M ;
VILLEGAS, E ;
REVAH, S .
BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (11) :1007-1013
[4]   Tannin acyl hydrolase (EC 3.1.1.20) activity of Aspergillus, Penicillium, Fusarium and Trichoderma [J].
Bajpai, B ;
Patil, S .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (03) :217-220
[5]   Induction of tannin acyl hydrolase (EC 3.1.1.20) activity in some members of fungi imperfecti [J].
Bajpai, B ;
Patil, S .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (08) :612-614
[6]  
BEVERINI M, 1990, SCI ALIMENT, V10, P807
[7]   Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus [J].
Bradoo, S ;
Gupta, R ;
Saxena, RK .
PROCESS BIOCHEMISTRY, 1997, 32 (02) :135-139
[8]  
Chatterjee R, 1996, BIOPROCESS ENG, V14, P159
[9]   Biomass estimation of Aspergillus niger growing on real and model supports in solid state fermentation [J].
CordovaLopez, J ;
GutierrezRojas, M ;
Huerta, S ;
SaucedoCastaneda, G ;
FavelaTorres, E .
BIOTECHNOLOGY TECHNIQUES, 1996, 10 (01) :1-6
[10]  
DESCHAMPS AM, 1983, J FERMENT TECHNOL, V61, P55