Platinum-catalyzed enzyme electrodes immobilized on gold using self-assembled layers

被引:245
作者
Gooding, JJ [1 ]
Praig, VG [1 ]
Hall, EAH [1 ]
机构
[1] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England
关键词
D O I
10.1021/ac971035t
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The bonding of enzymes to self-asembled monolayers (SAMs) of alkanethiols onto gold electrode surfaces is exploited to produce an enzyme biosensor, The attachment of glucose oxidase to a SAM of 3-mercaptopropionic acid was achieved using carbodiimide coupling. The resultant biosensor showed good sensitivity to glucose and a large dynamic range when measured amperometrically via the p-benzoquinone mediator. On the other hand, subsequent platinization of the enzyme-SAM: electrode allowed hydrogen peroxide produced in the enzyme reaction to be detected directly, thus obviating the need for an artificial redox mediator. The performance of such sensors constructed on bulk gold electrodes was evaluated and finally compared to that of some preliminary thin-film gold electrodes. Biosensors constructed using the two alternative electrode surfaces have quite different sensitivities, thus reflecting the influence of the anchoring surface on the performance of the biosensor.
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页码:2396 / 2402
页数:7
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