LC-MS-based methodology for global metabolite profiling in metabonomics/metabolomics

被引：289

作者：

Theodoridis, Georgios

Gika, Helen G.

Wilson, Ian D. ^{[1
]}

机构：

[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2008年 / 27卷 / 03期

关键词：

global metabolite profiling; metabolomics; metabonomics; metabotyping; separation; spectroscopic analysis;

D O I：

10.1016/j.trac.2008.01.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

in order to perform metabonomic investigations, it is necessary to generate comprehensive "global" metabolite profiles from complex samples, such as biofluids and tissues/tissue extracts. Analytical technologies based on liquid chromatography coupled to mass spectrometry (LC-MS) are destined to become a major source of global metabolite profiling data. Here, we describe and compare the utility of various analytical strategies and techniques currently used for LC-MS (capillary, high-performance and ultra-performance). We describe current challenges in the application of LC-MS-based techniques for metabonomic analysis. (c) 2008 Elsevier Ltd. All rights reserved.

引用

收藏

页码：251 / 260

页数：10

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