Chicken microchromosomes are hypermethylated and can be identified by specific painting probes

被引:25
作者
Grützner, F
Zend-Ajusch, E
Stout, K
Munsche, S
Niveleau, A
Nanda, I
Schmid, M
Haaf, T
机构
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
[2] Univ Grenoble 1, Mol & Struct Virol Unit, La Tronche, France
[3] Univ Wurzburg, Dept Human Genet, D-8700 Wurzburg, Germany
来源
CYTOGENETICS AND CELL GENETICS | 2001年 / 93卷 / 3-4期
关键词
D O I
10.1159/000056996
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes. Copyright (C) 2001 S. Karger Ag, Basel.
引用
收藏
页码:265 / 269
页数:5
相关论文
共 25 条
  • [1] AN EXTENDED CHICKEN KARYOTYPE, INCLUDING THE NOR CHROMOSOME
    AUER, H
    MAYR, B
    LAMBROU, M
    SCHLEGER, W
    [J]. CYTOGENETICS AND CELL GENETICS, 1987, 45 (3-4): : 218 - 221
  • [2] BLOOM SE, 1993, MANIPULATION OF THE AVIAN GENOME, P39
  • [3] Chudoba I, 1996, ONCOL REP, V3, P829
  • [4] Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH)
    Fillon, V
    Morisson, M
    Zoorob, R
    Auffray, C
    Douaire, M
    Gellin, J
    Vignal, A
    [J]. CHROMOSOME RESEARCH, 1998, 6 (04) : 307 - 313
  • [5] Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome
    Griffin, DK
    Haberman, F
    Masabanda, J
    O'Brien, P
    Bagga, M
    Sazanov, A
    Smith, J
    Burt, DW
    Ferguson-Smith, M
    Wienberg, J
    [J]. CYTOGENETICS AND CELL GENETICS, 1999, 87 (3-4): : 278 - 281
  • [6] Groenen MAM, 2000, GENOME RES, V10, P137
  • [7] Generation of whole-chromosome painting probes specific to each chicken macrochromosome
    Guillier-Gencik, Z
    Bernheim, A
    Coullin, P
    [J]. CYTOGENETICS AND CELL GENETICS, 1999, 87 (3-4): : 282 - 285
  • [8] HAAF T, 2000, ENCY ANAL CHEM, V1, P4984
  • [9] HISTONE ACETYLATION AND GLOBIN GENE SWITCHING
    HEBBES, TR
    THORNE, AW
    CLAYTON, AL
    CRANEROBINSON, C
    [J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (05) : 1017 - 1022
  • [10] Embryogenesis - Demethylation of the zygotic paternal genome
    Mayer, W
    Niveleau, A
    Walter, J
    Fundele, R
    Haaf, T
    [J]. NATURE, 2000, 403 (6769) : 501 - 502