Encapsulation of glucose oxidase and an oxygen-quenched fluorophore in polyelectrolyte-coated calcium alginate microspheres as optical glucose sensor systems

被引:87
作者
Brown, JQ
Srivastava, R
McShane, MJ
机构
[1] Louisiana Tech Univ, Biomed Engn Program, Ruston, LA 71272 USA
[2] Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71272 USA
关键词
polyelectrolyte multilayers; microspheres; optical glucose sensors;
D O I
10.1016/j.bios.2004.08.020
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Microspheres coated with polyelectrolyte multilayers (PEM's) are being investigated for potential use as implantable biosensors-so-called "smart tattoos." In this work, the feasibility of this approach for glucose sensors was demonstrated by glucose oxidase encapsulated within calcium alginate microspheres, followed by entrapment of an oxygen-quenched ruthenium compound in the same microstructure. A novel feature of these microdevices is the formation of multilayer nanofilms on the surface of the microspheres, used to stabilize enzyme entrapment and control substrate diffusion. Confocal microscopy was used to confirm the stable encapsulation of sensor chemistry. The reversible response of sensors to step changes in glucose was observed, and preliminary experimental data were compared to theoretical predictions produced by a computational model. These findings demonstrate the promise of the described nanoengineering approach for production of functional implantable glucose sensor materials. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:212 / 216
页数:5
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