A two-step sulfurization for efficient solution-phase synthesis of phosphorothioate oligonucleotides

被引：6

作者：

Ghisaidoobe, Amar B. T. ^{[1
]}

de Koning, Martijn C. ^{[1
]}

Duynstee, Howard I. ^{[2
]}

Ten Kortenaar, Paul B. W. ^{[2
]}

Overkleeft, Herman S. ^{[1
]}

Filippov, Dmitri V. ^{[1
]}

van der Marel, Gijs A. ^{[1
]}

机构：

[1] Gorlaeus Labs, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands

[2] Diosynth Int BV, NL-5340 BH Oss, Netherlands

来源：

TETRAHEDRON LETTERS | 2008年 / 49卷 / 19期

关键词：

D O I：

10.1016/j.tetlet.2008.03.025

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A solution-phase approach for the synthesis of phosphorothioate oligonucleotides that circumvents the use of chromatographic purifications of protected phosphorothioate intermediates was developed. Implementation of a two-step sulfurization protocol in the phosphoramidite method allows efficient isolation of the intermediate phosphorothioates by extractions. The viability of this approach is demonstrated by the synthesis of a hexameric phosphorothioate oligonucleotide fragment. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：3129 / 3132

页数：4

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