Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

被引：182

作者：

Wang, Feng

Guo, Chen ^{[1
]}

Yang, Liang-rong

Liu, Chun-Zhao ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 23期

基金：

北京市自然科学基金; 中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Immobilization; Laccase; Mesoporous; Nanoparticles; PYCNOPORUS-SANGUINEUS LACCASE; TRAMETES-VERSICOLOR LACCASE; CORIOLUS-VERSICOLOR; PURIFICATION; ADSORPTION; BEADS; DECOLORIZATION; PARTICLES; CARRIERS;

D O I：

10.1016/j.biortech.2010.06.115

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Newly large-pore magnetic mesoporous silica nanoparticles (MMSNPs) with wormhole framework structures were synthesized for the first time by using tetraethyl orthosilicate as the silica source and amine-terminated Jeffamine surfactants as template. Iminodiacerate was attached on these MMSNPs through a silane-coupling agent and chelated with Cu2+. The Cu2+-chelated MMSNPs (MMSNPs-CPTS-IDA-Cu2+) showed higher adsorption capacity of 98.1 mg g(-1)-particles and activity recovery of 92.5% for laccase via metal affinity adsorption in comparison with MMSNPs via physical adsorption. The Michaelis constant (K-m) and catalytic constant (k(cat)) of laccase immobilized on the MMSNPs-CPTS-IDA-Cu2+ were 3.28 mM and 155.4 min(-1), respectively. Storage stability and temperature endurance of the immobilized laccase on MMSNPs-CPTS-IDA-Cu2+ increased significantly, and the immobilized laccase retained 86.6% of its initial activity after 10 successive batch reactions operated with magnetic separation. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：8931 / 8935

页数：5

共 35 条

[1] Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation [J].

Arica, M. Yakup ;

Altintas, Begum ;

Bayramoglu, Guelay .

BIORESOURCE TECHNOLOGY, 2009, 100 (02) :665-669

[2] Study on synthesis of a hydrophilic bead carrier containing epoxy groups. and its proper-ties for glucoamylase immobilization [J].

Bai, Yong-Xiao ;

Li, Yan-Feng ;

Wang, Ming-Tao .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 39 (04) :540-547

[3] Immobilization of laccase onto poly(glycidylmethacrylate) brush grafted poly(hydroxyethylmethacrylate) films: Enzymatic oxidation of phenolic compounds [J].

Bayramoglu, Guelay ;

Arica, M. Yakup .

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2009, 29 (06) :1990-1997

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Immobilization of laccase from the white rot fungus Coriolopsis polyzona and use of the immobilized biocatalyst for the continuous elimination of endocrine disrupting chemicals [J].

Cabana, Hubert ;

Alexandre, Celine ;

Agathos, Spiros N. ;

Jones, J. Peter .

BIORESOURCE TECHNOLOGY, 2009, 100 (14) :3447-3458

[6] Industrial and biotechnological applications of laccases: A review [J].

Couto, Susana Rodriguez ;

Herrera, Jose Luis Toca .

BIOTECHNOLOGY ADVANCES, 2006, 24 (05) :500-513

[7]

DAVIS S, 1992, APPL MICROBIOL BIOT, V37, P474, DOI 10.1007/BF00180972

[8] Production of laccase and xylanase from Coriolus versicolor grown on tomato pomace and their chromatographic behaviour on immobilized metal chelates [J].

do Rosario Freixo, Maria ;

Karmali, Amin ;

Frazao, Carlos ;

Maria Arteiro, Jose .

PROCESS BIOCHEMISTRY, 2008, 43 (11) :1265-1274

[9] PURIFICATION OF ALPHA-1-PROTEINASE INHIBITOR BY TRIAZINE DYE AFFINITY-CHROMATOGRAPHY, ION-EXCHANGE CHROMATOGRAPHY AND GEL-FILTRATION ON FRACTOGEL TSK [J].

GUNZER, G ;

HENNRICH, N .

JOURNAL OF CHROMATOGRAPHY, 1984, 296 (JUL) :221-229

[10] Catalytic activity of mesoporous silicate-immobilized chloroperoxidase [J].

Han, YJ ;

Watson, JT ;

Stucky, GD ;

Butler, A .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 17 (01) :1-8

← 1 2 3 4 →