RNA silencing platforms in plants

被引：123

作者：

Watson, JM ^{[1
]}

Fusaro, AF ^{[1
]}

Wang, MB ^{[1
]}

Waterhouse, PM ^{[1
]}

机构：

[1] CSIRO, Plant Ind, Canberra, ACT 2602, Australia

来源：

FEBS LETTERS | 2005年 / 579卷 / 26期

关键词：

RNAi; VIGS; hpRNA; shRNA;

D O I：

10.1016/j.febslet.2005.08.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and concentrate on discussing the more recent advances, comparing the RNAi technologies used in plants with those used in animals, and trying to predict the ways in which RNAi technologies may further develop. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：5982 / 5987

页数：6

相关论文

共 56 条

[11] Viral delivery of recombinant short hairpin RNAs [J].

Davidson, BL ;

Harper, SQ .

RNA INTERFERENCE, 2005, 392 :145-173

[12] A geminivirus-induced gene silencing system for gene function validation in cassava [J].

Fofana, IBF ;

Sangaré, A ;

Collier, R ;

Taylor, C ;

Fauquet, CM .

PLANT MOLECULAR BIOLOGY, 2004, 56 (04) :613-624

[13] SVISS -: a novel transient gene silencing system for gene function discovery and validation in tobacco plants [J].

Gosselé, V ;

Faché, I ;

Meulewaeter, F ;

Cornelissen, M ;

Metzlaff, M .

PLANT JOURNAL, 2002, 32 (05) :859-866

[14] A chemical-regulated inducible RNAi system in plants [J].

Guo, HS ;

Fei, JF ;

Xie, Q ;

Chua, NH .

PLANT JOURNAL, 2003, 34 (03) :383-392

[15] Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley [J].

Hein, I ;

Pacak, MB ;

Hrubikova, K ;

Williamson, S ;

Dinesen, M ;

Soenderby, IE ;

Sundar, S ;

Jarmolowski, A ;

Shirasu, K ;

Lacomme, C .

PLANT PHYSIOLOGY, 2005, 138 (04) :2155-2164

[16] Constructs and methods for hairpin RNA-mediated gene silencing in plants [J].

Helliwell, CA ;

Waterhouse, PM .

RNA INTERFERENCE, 2005, 392 :24-35

[17] High-throughput vectors for efficient gene silencing in plants [J].

Helliwell, CA ;

Wesley, SV ;

Wielopolska, AJ ;

Waterhouse, PM .

FUNCTIONAL PLANT BIOLOGY, 2002, 29 (10) :1217-1225

[18] European consortia building integrated resources for Arabidopsis functional genomics [J].

Hilson, P ;

Small, I ;

Kuiper, MTR .

CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (05) :426-429

[19] Barley stripe mosaic virus-induced gene silencing in a monocot plant [J].

Holzberg, S ;

Brosio, P ;

Gross, C ;

Pogue, GP .

PLANT JOURNAL, 2002, 30 (03) :315-327

[20] Expression profiling reveals off-target gene regulation by RNAi [J].

Jackson, AL ;

Bartz, SR ;

Schelter, J ;

Kobayashi, SV ;

Burchard, J ;

Mao, M ;

Li, B ;

Cavet, G ;

Linsley, PS .

NATURE BIOTECHNOLOGY, 2003, 21 (06) :635-637

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