A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica

被引：101

作者：

Kim, J ^{[1
]}

Lee, J

Na, HB

Kim, BC

Youn, JK

Kwak, JH

Moon, K

Lee, E

Kim, J ^{[1
]}

Park, J

Dohnalkova, A

Park, HG

Gu, MB

Chang, HN

Grate, JW

Hyeon, T

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

[2] Seoul Natl Univ, Natl Creat Res Initiat Ctr Oxide Nanocrystalline, Seoul 151744, South Korea

[3] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151744, South Korea

[4] Gwangju Inst Sci & Technol, Adv Environm Monitoring Res Ctr, Kwangju 500712, South Korea

[5] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea

[6] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea

来源：

SMALL | 2005年 / 1卷 / 12期

关键词：

enzyme catalysis; magnetic nanoparticles; magnetic separation; mesoporous materials; silica;

D O I：

10.1002/smll.200500245

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The development of a magnetically separable and highly stable enzyme system, using nanoparticles and mesoporous silica, was investigated. Nanometer-scale composites of enzyme molecules and hierarchically ordered magnetite nanoparticles were immobilized via a ship-in-a-bottle approach, which used co-adsorption of enzymes and magnetite (Fe3O4) nanoparticles. The enzyme molecules were crosslinked via glutaraldehyde (GA) treatment resulting in the effective entrapment of neighboring magnetite nanoparticles. These nanocomposites, called M-CLEAs, were found magnetically separable, highly loaded with enzymes, stable under harsh conditions, resistant to proteolytic digestion, and recyclable for iterative use with negligible loss of enzyme activity. The ship-in-a-bottle approach can be expanded to many other enzymes and has applications in bioremediation and bioconversion.

引用

页码：1203 / 1207

页数：5

共 46 条

[31] Industrial biocatalysis today and tomorrow [J].