A multiple-capillary electrophoresis system for smallscale DNA sequencing and analysis

被引：61

作者：

Zhang, Jianzhong ^{[1
]}

Voss, Karl O. ^{[1
]}

Shaw, Diana F. ^{[1
]}

Roos, K. Pieter ^{[1
]}

Lewis, Darren F. ^{[1
]}

Yan, Juying ^{[1
]}

Jiang, Rong ^{[1
]}

Ren, Hongji ^{[1
]}

Hou, Joan Y. ^{[1
]}

Fang, Yu ^{[1
]}

Puyang, Xiaoling ^{[1
]}

Ahmadzadeh, Hossein ^{[1
]}

Dovichi, Norman J. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 24期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/27.24.e36

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A five-capillary system has been developed for DNA sequencing and analysis. The post-column fluorescence detector is based on a sheath-flow cuvette. The instrument provides uniform and continuous illumination of the samples. The cuvette virtually eliminates cross-talk in the fluorescence signal between capillaries. Discrete single-photon counting avalanche photodiodes provide high efficiency light detection. The instrument has detection limits (3 sigma) of 130 +/- 30 fluorescein molecules injected onto each capillary. Over 650 bases of sequence at 98.8% accuracy were generated in 100 min at 50 degrees C from M13mp18. Separation and detection of short tandem repeats proved efficient and accurate with the use of internal standards for direct comparison of migration times between capillaries.

引用

页数：7

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