Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma

被引：25

作者：

Deng, Z. ^{[1
,2
]}

Wang, Y. ^{[2
,3
]}

Fang, X. ^{[1
,2
]}

Yan, F. ^{[2
,4
]}

Pan, H. ^{[1
,2
]}

Gu, L. ^{[1
,2
]}

Xie, C. ^{[1
,2
]}

Li, Y. ^{[2
,4
]}

Hu, Y. ^{[1
,2
]}

Cao, Y. ^{[5
]}

Tang, Z. ^{[1
,2
]}

机构：

[1] Cent S Univ, Dept Oral & Maxillofacial Surg, 72 Xiangya Rd, Changsha 410008, Hunan, Peoples R China

[2] Xiangya Stomatol Hosp, 72 Xiangya Rd, Changsha 410008, Hunan, Peoples R China

[3] Cent S Univ, Dept Dent Implant, Changsha, Hunan, Peoples R China

[4] Cent S Univ, Dept Prosthodont, Changsha, Hunan, Peoples R China

[5] Xinjiang Med Univ, Affiliated Hosp 1, Dept Prosthodont, Changsha, Hunan, Peoples R China

来源：

CANCER GENE THERAPY | 2017年 / 24卷 / 07期

关键词：

SQUAMOUS-CELL CARCINOMA; SUPPRESSES TUMORIGENICITY; HEPATOCELLULAR-CARCINOMA; CANCER CELLS; LUNG-CANCER; EXPRESSION; MICRORNAS; CAVITY;

D O I：

10.1038/cgt.2017.18

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Oral verrucous carcinoma (OVC) is a verrucous variant of oral cavity squamous carcinoma (OSCC). The expression of miRNA from OVC and OSCC including their matched normal oral mucosa tissues was profiled through the Affymetrix GeneChip miRNA Arrays. TargetScan and miRanda databases were used to predict the target gene of miRNA-195. The quantitative real-time PCR was applied to validate the expression of miRNA-195. The expression of CDK6 was investigated by the quantitative real-time PCR and immunohistochemistry. In this study, a total of 23 and 35 differentially expressed miRNAs were identified in OVC and OSCC, respectively. Moreover, 44 miRNAs were differentially expressed between OSCC and OVC. In addition, miRNA-195 was significantly decreased in both OVC and OSCC compared to normal oral mucosa. Target gene prediction demonstrated that CDK6 was a potential target gene of miRNA-195. In the quantitative real-time PCR, miR-195 was decreased in OVC and OSCC, which was consistent with the result of miRNA chip analysis. CDK6 was increased in OVC and OSCC, which was opposite to the expression of miRNA-195. In conclusion, miRNA-195 could be the potential diagnosis biomarker and therapy target of OVC.

引用

页码：282 / 288

页数：7

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