Isolation and purification of coumarin compounds from Cortex fraxinus by high-speed counter-current chromatography

被引：102

作者：

Liu, RM ^{[1
]}

Sun, QH ^{[1
]}

Sun, AL ^{[1
]}

Cui, JC ^{[1
]}

机构：

[1] Liaocheng Univ, Coll Chem & Chem Engn, Liaocheng 252059, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1072卷 / 02期

关键词：

counter-current chromatography; Cortex fraxinus; fraxin; aesculin; fraxetin; aesculetin;

D O I：

10.1016/j.chroma.2005.03.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of coumarin compounds from Cortex fraxinus, the Chinese herbal drug. n-Butanol-methanol-0.5% acetic acid (5:1.5:5, v/v) was used as the two-phase solvent system. 14.3 mg of fraxin, 26.5 mg of aesculin, 5.8 mg of fraxetin and 32.4 mg of aesculetin with the purity of 97.6, 99.5, 97.2 and 98.7 %, respectively were obtained from 150 mg of crude extracts of C. fraxinus in a single run. The structures of the isolated compounds were identified by H-1 NMR and C-13 NMR. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：195 / 199

页数：5

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