Application of nanostructured ZnO films for electrochemical DNA biosensor

被引：67

作者：

Das, Maumita ^{[1
]}

Sumana, Gajjala ^{[1
]}

Nagarajan, R. ^{[2
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] CSIR, Natl Phys Lab, Mat Phys & Engn Div,Dept Sci & Technol, Biomed Instrumentat Sect,Ctr Biomol Elect, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, Delhi 110007, India

来源：

THIN SOLID FILMS | 2010年 / 519卷 / 03期

关键词：

Zinc oxide (ZnO); DNA biosensor; Electrochemical deposition; MYCOBACTERIUM-TUBERCULOSIS DETECTION; NUCLEIC-ACID SENSOR; ZINC-OXIDE; ELECTRODES;

D O I：

10.1016/j.tsf.2010.08.069

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium-tin-oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA-nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 x 10(-6) - 1 x 10(-12) M, detection limit of 1 x 10(-12) M (complementary target) and 1 x 10(-13) M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4 degrees C. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1196 / 1201

页数：6

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