Microchip capillary gel electrophoresis using programmed field strength gradients for the ultra-fast analysis of genetically modified organisms in soybeans

被引：21

作者：

Kim, YJ

Chae, JS

Chang, JK

Kang, SH ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Dept Chem, Jeoju 561756, South Korea

[2] Chonbuk Natl Univ, Biosafety Res Inst, Jeoju 561756, South Korea

[3] Chonbuk Natl Univ, Coll Vet Med, Jeoju 561756, South Korea

[4] SKC Cent Res Inst, Digital Bio Technol, Suwon 440301, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1083卷 / 1-2期

关键词：

chip technology; GMO; fast analysis; programmed field strength gradients;

D O I：

10.1016/j.chroma.2005.06.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a novel method for the ultra-fast analysis of genetically modified organisms (GMOs) in soybeans by microchip capillary gel electrophoresis (MCGE) using programmed field strength gradients (PFSG) in a conventional glass double-T microchip. Under the programmed electric field strength and 0.3% poly(ethylene oxide) sieving matrix, the GMO in soybeans was analyzed within only 11 s of the microchip. The MCGE-PFSG method was a program that changes the electric field strength during GMO analysis, and was also applied to the ultra-fast analysis of PCR products. Compared to MCGE using a conventional and constantly applied electric field, the MCGE-PFSG analysis generated faster results without the loss of resolving power and reproducibility for specific DNA fragments (100- and 250-bp DNA) of GM-soybeans. The MCGE-PFSG technique may prove to be a new tool in the GMO analysis due to its speed, simplicity, and high efficiency. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：179 / 184

页数：6

共 27 条

[21] Rapid analysis of genetically modified organisms by in-house developed capillary electrophoresis chip and laser-induced fluorescence system [J].