Analysis of fentanyl and norfentanyl in human plasma by liquid chromatography-tandem mass spectrometry using electrospray ionization

被引：37

作者：

Day, J ^{[1
]}

Slawson, M ^{[1
]}

Lugo, RA ^{[1
]}

Wilkins, D ^{[1
]}

机构：

[1] Univ Utah, Ctr Human Toxicol, Salt Lake City, UT 84112 USA

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2003年 / 27卷 / 07期

关键词：

D O I：

10.1093/jat/27.7.513

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This report describes a sensitive and specific high-performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry (MS-MS) method for the detection of subnanogram concentrations of fentanyl and its metabolite norfentanyl in human plasma. The assay was based on a liquid-liquid extraction of 0.5 mL of human plasma, with a lower limit of quantitation (LLOQ) of 0.05 ng/mL. Sample extracts were analyzed using a ThermoQuest TSQ® MS-MS interfaced with a Hewlett-Packard series 1100 HPLC and a Phenomenex® (30 × 2.00-mm, 5 μ Luna C18(2)) column. The intra-assay precision and accuracy ranged from 2.1 to 12.5% for both analytes at concentrations of 0.1, 0.5, 1.0, and 10 ng/mL. The interassay accuracy and precision ranged from 7.34 to 10.95%.

引用

页码：513 / 516

页数：4

共 13 条

[1] Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection [J].

Choi, HS ;

Shin, HC ;

Khang, G ;

Rhee, JM ;

Lee, HB .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2001, 765 (01) :63-69

[2] Determination of subnanogram concentrations of fentanyl in plasma by gas chromatography mass spectrometry: Comparison with standard radioimmunoassay [J].

Fryirsa, B ;

Woodhouse, A ;

Huang, JL ;

Dawson, M ;

Mather, LE .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1997, 688 (01) :79-85

[3]

GOODMAN AG, 1996, PHARMACOL BASIS THER, P543

[4] DETERMINATION OF FENTANYL AND ALFENTANIL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION [J].

KUMAR, K ;

MORGAN, DJ ;

CRANKSHAW, DP .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 419 :464-468

[5] Development and validation of an HPLC assay for fentanyl and related substances in fentanyl citrate injection, USP [J].

Lambropoulos, J ;

Spanos, GA ;

Lazaridis, NV ;

Ingallinera, TS ;

Rodriguez, VK .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 20 (04) :705-716

[6] DETERMINATION OF PLASMA FENTANYL BY GC-MASS SPECTROMETRY AND PHARMACOKINETIC ANALYSIS [J].

LIN, SN ;

WANG, TPF ;

CAPRIOLI, RM ;

MO, BPN .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1981, 70 (11) :1276-1279

[7] MEASUREMENT OF PLASMA FENTANYL CONCENTRATION - COMPARISON OF 3 METHODS [J].

PHIPPS, JA ;

SABOURIN, MA ;

BUCKINGHAM, W ;

STRUNIN, L .

CANADIAN ANAESTHETISTS SOCIETY JOURNAL, 1983, 30 (02) :162-165

[8] DETECTION OF PICOGRAM CONCENTRATIONS OF FENTANYL IN PLASMA BY GAS-LIQUID-CHROMATOGRAPHY [J].

PHIPPS, JA ;

SABOURIN, MA ;

BUCKINGHAM, W ;

STRUNIN, L .

JOURNAL OF CHROMATOGRAPHY, 1983, 272 (02) :392-395

[9] OPTIMIZATION OF THE RADIOIMMUNOASSAYS FOR MEASURING FENTANYL AND ALFENTANIL IN HUMAN-SERUM [J].

SCHUTTLER, J ;

WHITE, PF .

ANESTHESIOLOGY, 1984, 61 (03) :315-320

[10] A highly automated 96-well solid phase extraction and liquid chromatography/tandem mass spectrometry method for the determination of fentanyl in human plasma [J].

Shou, WZ ;

Jiang, XY ;

Beato, BD ;

Weng, ND .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (07) :466-476

← 1 2 →