Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS

被引：335

作者：

Lee, Jung-Shin ^{[1
]}

Shukla, Abhijit ^{[2
]}

Schneider, Jessica ^{[1
]}

Swanson, Selene K. ^{[1
]}

Washburn, Michael P. ^{[1
]}

Florens, Laurence ^{[1
]}

Bhaumik, Sukesh R. ^{[2
]}

Shilatifard, Ali ^{[1
]}

机构：

[1] Stowers Inst Med Res, Kansas City, MO 64110 USA

[2] So Illinois Univ, Sch Med, Dept Biochem & Mol Biol, Carbondale, IL 62901 USA

来源：

CELL | 2007年 / 131卷 / 06期

关键词：

D O I：

10.1016/j.cell.2007.09.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

COMPASS, the yeast homolog of the mammalian MLL complex, is a histone H3 lysine 4 (H3K4) methylase consisting of Set1 (KMT2) and seven other polypeptides, including Cps35, the only essential subunit. Histone H2B monoubiquitination by Rad6/Bre1 is required for both H3K4 methylation by COMPASS, and H3K79 methylation by Dot1. However, the molecular mechanism for such histone crosstalk is poorly understood. Here, we demonstrate that histone H2B monoubiquitination controls the binding of Cps35 with COMPASS complex. Cps 35 is required for COMPASS' catalytic activity in vivo, and the addition of exogenous purified Cps35 to COMPASS purified from a Delta rad6 background results in the generation of a methylation competent COMPASS. Cps35 associates with the chromatin of COMPASS-regulated genes in a H2BK123 monoubiquitination-dependent but Set1-independent manner. Cps35 is also required for proper H3K79 trimethylation. These findings offer insight into the molecular role of Cps35 in translating the H2B monoubiquitination signal into H3 methylation.

引用

收藏

页码：1084 / 1096

页数：13

相关论文

共 59 条

[11] Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors [J].

Florens, Laurence ;

Carozza, Michael J. ;

Swanson, Selene K. ;

Fournier, Marjorie ;

Coleman, Michael K. ;

Workman, Jerry L. ;

Washburn, Michael P. .

METHODS, 2006, 40 (04) :303-311

[12] SUMO and ubiquitin in the nucleus: different functions, similar mechanisms? [J].

Gill, G .

GENES & DEVELOPMENT, 2004, 18 (17) :2046-2059

[13] Menin associates with a trithorax family histone methyltransferase complex and with the Hoxc8 locus [J].

Hughes, CM ;

Rozenblatt-Rosen, O ;

Milne, TA ;

Copeland, TD ;

Levine, SS ;

Lee, JC ;

Hayes, DN ;

Shanmugam, KS ;

Bhattacharjee, A ;

Biondi, CA ;

Kay, GF ;

Hayward, NK ;

Hess, JL ;

Meyerson, M .

MOLECULAR CELL, 2004, 13 (04) :587-597

[14] The human homolog of yeast BRE1 functions as a transcriptional coactivator through direct activator interactions [J].

Kim, J ;

Hake, SB ;

Roeder, RG .

MOLECULAR CELL, 2005, 20 (05) :759-770

[15] The Paf1 complex is required for histone h3 methylation by COMPASS and Dot1p: Linking transcriptional elongation to histone methylation [J].

Krogan, NJ ;

Dover, J ;

Wood, A ;

Schneider, J ;

Heidt, J ;

Boateng, MA ;

Dean, K ;

Ryan, OW ;

Golshani, A ;

Johnston, M ;

Greenblatt, JF ;

Shilatifard, A .

MOLECULAR CELL, 2003, 11 (03) :721-729

[16] COMPASS, a histone H3 (lysine 4) methyltransferase required for telomeric silencing of gene expression [J].

Krogan, NJ ;

Dover, J ;

Khorrami, S ;

Greenblatt, JF ;

Schneider, J ;

Johnston, M ;

Shilatifard, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (13) :10753-10755

[17] Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase [J].

Lacoste, N ;

Utley, RT ;

Hunter, JM ;

Poirier, GG ;

Côté, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (34) :30421-30424

[18] BUR kinase selectively regulates H3K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex [J].

Laribee, RN ;

Krogan, NJ ;

Xiao, TJ ;

Shibata, Y ;

Hughes, TR ;

Greenblatt, JF ;

Strahl, BD .

CURRENT BIOLOGY, 2005, 15 (16) :1487-1493

[19] The role of chromatin during transcription [J].

Li, Bing ;

Carey, Michael ;

Workman, Jerry L. .

CELL, 2007, 128 (04) :707-719

[20] The Chd family of chromatin remodelers [J].

Marfella, Concetta G. A. ;

Imbalzano, Anthony N. .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2007, 618 (1-2) :30-40

← 1 2 3 4 5 6 →