Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays

被引：35

作者：

Fernandez-Arguelles, M. T. ^{[1
]}

Costa-Fernandez, J. M. ^{[1
]}

Pereiro, R. ^{[1
]}

Sanz-Medel, A. ^{[1
]}

机构：

[1] Univ Oviedo, Dept Phys & Analyt Chem, Oviedo 33006, Spain

来源：

ANALYST | 2008年 / 133卷 / 04期

关键词：

D O I：

10.1039/b802360n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.

引用

页码：444 / 447

页数：4

共 21 条

[1] The use of nanocrystals in biological detection [J].

Alivisatos, P .

NATURE BIOTECHNOLOGY, 2004, 22 (01) :47-52

[2] Quantum dots in biology and medicine [J].

Bailey, RE ;

Smith, AM ;

Nie, SM .

PHYSICA E-LOW-DIMENSIONAL SYSTEMS & NANOSTRUCTURES, 2004, 25 (01) :1-12

[3] The use of luminescent quantum dots for optical sensing [J].

Costa-Fernández, JM ;

Pereiro, R ;

Sanz-Medel, A .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2006, 25 (03) :207-218

[4] Synthesis and characterization of polymer-coated quantum dots with integrated acceptor dyes as FRET-based nanoprobes [J].

Fernandez-Argueelles, Maria Teresa ;

Yakovlev, Aleksey ;

Sperling, Ralph A. ;

Luccardini, Camilla ;

Gaillard, Stephane ;

Medel, Alfredo Sanz ;

Mallet, Jean-Maurice ;

Brochon, Jean-Claude ;

Feltz, Anne ;

Oheim, Martin ;

Parak, Wolfgang J. .

NANO LETTERS, 2007, 7 (09) :2613-2617

[5] Multiplexed toxin analysis using four colors of quantum dot fluororeagents [J].

Goldman, ER ;

Clapp, AR ;

Anderson, GP ;

Uyeda, HT ;

Mauro, JM ;

Medintz, IL ;

Mattoussi, H .

ANALYTICAL CHEMISTRY, 2004, 76 (03) :684-688

[6] Luminescent quantum dots in immunoassays [J].

Goldman, ER ;

Medintz, IL ;

Mattoussi, H .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (03) :560-563

[7] Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins [J].

Goldman, ER ;

Medintz, IL ;

Hayhurst, A ;

Anderson, GP ;

Mauro, JM ;

Iverson, BL ;

Georgiou, G ;

Mattoussi, H .

ANALYTICA CHIMICA ACTA, 2005, 534 (01) :63-67

[8] Detection of proteins cross-linked within galactoside polyacrylate-based hydrogels by means of a quantum dot fluororeagent [J].

Goldman, ER ;

O'Shaughnessy, TJ ;

Soto, CM ;

Patterson, CH ;

Taitt, CR ;

Spector, MS ;

Charles, PT .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 380 (7-8) :880-886

[9] Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays [J].

Goldman, ER ;

Anderson, GP ;

Tran, PT ;

Mattoussi, H ;

Charles, PT ;

Mauro, JM .

ANALYTICAL CHEMISTRY, 2002, 74 (04) :841-847

[10] Avidin: A natural bridge for quantum dot-antibody conjugates [J].

Goldman, ER ;

Balighian, ED ;

Mattoussi, H ;

Kuno, MK ;

Mauro, JM ;

Tran, PT ;

Anderson, GP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) :6378-6382

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