Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane

被引:32
作者
Saurina, J
Hernández-Cassou, S
Fàbregas, E
Alegret, S
机构
[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain
[2] Univ Autonoma Barcelona, Dept Chem, E-08193 Barcelona, Spain
关键词
potentiometric lysine biosensor; immobilized lysine oxidase membranes; all-solid-state ammonium electrode; lysine analysis;
D O I
10.1016/S0003-2670(98)00310-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An enzymatic lysine electrode is developed for the potentiometric analysis of lysine (Lys). This lysine biosensor consists of a chemically immobilized lysine oxidase membrane attached to an all-solid-state ammonium electrode. In this system, the enzymatic degradation of lysine produces ammonia, which is then detected with the ammonium electrode. The influence of immobilization conditions on the potentiometric response and the lifetime of the electrode was studied. Under optimal conditions, the relationship between the potentiometric response and the log [Lys] was linear between 3x10(-5) and 10(-3) M, the sensitivity was 50 mV/decade, the repeatability 1.4%, and the response time t(95%) was 15 s. (C) 1998 Elsevier Science B.V, All rights reserved.
引用
收藏
页码:49 / 56
页数:8
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