High-resolution solid-state NMR applied to polypeptides and membrane proteins

被引：88

作者：

Luca, S ^{[1
]}

Heise, H ^{[1
]}

Baldus, M ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, Solid State NMR, D-37077 Gottingen, Germany

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2003年 / 36卷 / 11期

关键词：

D O I：

10.1021/ar020232y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Solid-state NMR provides unique possibilities to study insoluble or noncrystalline molecules at the atomic level. High-resolution conditions can be established by employing magic-angle spinning at ultrahigh magnetic fields. We discuss NMR methods that make use of these experimental improvements and allow for the study of multiply or uniformly [C-13,N-15]-labeled polypeptides and proteins. Recent biophysical applications are reviewed.

引用

收藏

页码：858 / 865

页数：8

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