Time-resolved protein nanocrystallography using an X-ray free-electron laser

被引：184

作者：

Aquila, Andrew ^{[1
]}

Hunter, Mark S. ^{[2
]}

Doak, R. Bruce ^{[3
]}

Kirian, Richard A. ^{[3
]}

Fromme, Petra

White, Thomas A. ^{[1
]}

Andreasson, Jakob ^{[4
]}

Arnlund, David

Bajt, Sasa

Barends, Thomas R. M. ^{[5
,6
]}

Barthelmess, Miriam

Bogan, Michael J. ^{[7
]}

Bostedt, Christoph ^{[8
]}

Bottin, Herve ^{[9
]}

Bozek, John D. ^{[8
]}

Caleman, Carl ^{[1
]}

Coppola, Nicola ^{[10
]}

Davidsson, Jan

DePonte, Daniel P. ^{[1
]}

Elser, Veit ^{[11
]}

Epp, Sascha W. ^{[5
,12
]}

Erk, Benjamin ^{[5
,12
]}

Fleckenstein, Holger ^{[1
]}

Foucar, Lutz ^{[5
,6
]}

Frank, Matthias

Fromme, Raimund

Graafsma, Heinz

Grotjohann, Ingo

Gumprecht, Lars

Hajdu, Janos

Hampton, Christina Y. ^{[7
]}

Hartmann, Andreas ^{[14
]}

Hartmann, Robert ^{[14
]}

Hauriege, Stefan ^{[13
]}

Hauser, Guenter ^{[15
]}

Hirsemann, Helmut

Holl, Peter

Holton, James M. ^{[16
]}

Hoemke, Andre ^{[5
,12
]}

Johansson, Linda

Kimmel, Nils

Kassemeyer, Stephan ^{[6
]}

Krasniqi, Faton ^{[6
]}

Kuehnel, Kaiuwe ^{[12
]}

Liang, Mengning

Lomb, Lukas ^{[6
]}

Malmerberg, Erik

Marchesini, Stefano ^{[16
]}

Martin, Andrew V.

Maia, Filipe R. N. C. ^{[4
]}

机构：

[1] DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[2] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[3] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[4] Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, SE-75124 Uppsala, Sweden

[5] CFEL, Max Planck Adv Study Grp, D-22607 Hamburg, Germany

[6] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

[7] PULSE Inst, Menlo Pk, CA 94025 USA

[8] SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA

[9] CEA, Inst Biol & Technol Saclay, F-91191 Gif Sur Yvette, France

[10] European XFEL GmbH, D-22761 Hamburg, Germany

[11] Cornell Univ, Dept Phys, Ithaca, NY 14853 USA

[12] Max Planck Inst Kernphys, D-69117 Heidelberg, Germany

[13] Lawrence Livermore Natl Lab, Livermore, CA 94551 USA

[14] PNSensor GmbH, D-81739 Munich, Germany

[15] Max Planck Inst Halbleiterlab, D-81739 Munich, Germany

[16] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Adv Light Source, Berkeley, CA 94720 USA

[17] Univ Hamburg, D-22761 Hamburg, Germany

[18] Max Planck Inst Extraterr Phys, D-85741 Garching, Germany

来源：

OPTICS EXPRESS | 2012年 / 20卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院; 瑞典研究理事会;

关键词：

PHOTOSYSTEM-I; STRUCTURAL DYNAMICS; CRYSTALLOGRAPHY; FERREDOXIN; CRYSTALLIZATION; DIFFRACTION; FLAVODOXIN; REDUCTION;

D O I：

10.1364/OE.20.002706

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 mu s after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems. (C) 2012 Optical Society of America

引用

页码：2706 / 2716

页数：11

共 30 条

[11]

Emma P, 2010, NAT PHOTONICS, V4, P641, DOI [10.1038/nphoton.2010.176, 10.1038/NPHOTON.2010.176]

[12] Crystallization and electron paramagnetic resonance characterization of the complex of photosystem I with its natural electron acceptor ferredoxin [J].

Fromme, P ;

Bottin, H ;

Krauss, N ;

Sétif, P .

BIOPHYSICAL JOURNAL, 2002, 83 (04) :1760-1773

[13] Improved isolation and crystallization of Photosystem I for structural analysis [J].

Fromme, P ;

Witt, HT .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1365 (1-2) :175-184

[14] Time-resolved pump-probe experiments at the LCLS [J].

Glownia, James M. ;

Cryan, J. ;

Andreasson, J. ;

Belkacem, A. ;

Berrah, N. ;

Blaga, C. I. ;

Bostedt, C. ;

Bozek, J. ;

DiMauro, L. F. ;

Fang, L. ;

Frisch, J. ;

Gessner, O. ;

Guehr, M. ;

Hajdu, J. ;

Hertlein, M. P. ;

Hoener, M. ;

Huang, G. ;

Kornilov, O. ;

Marangos, J. P. ;

March, A. M. ;

McFarland, B. K. ;

Merdji, H. ;

Petrovic, V. S. ;

Raman, C. ;

Ray, D. ;

Reis, D. A. ;

Trigo, M. ;

White, J. L. ;

White, W. ;

Wilcox, R. ;

Young, L. ;

Coffee, R. N. ;

Bucksbaum, P. H. .

OPTICS EXPRESS, 2010, 18 (17) :17620-17630

[15] BioCARS: a synchrotron resource for time-resolved X-ray science [J].

Graber, T. ;

Anderson, S. ;

Brewer, H. ;

Chen, Y. -S. ;

Cho, H. S. ;

Dashdorj, N. ;

Henning, R. W. ;

Kosheleva, I. ;

Macha, G. ;

Meron, M. ;

Pahl, R. ;

Ren, Z. ;

Ruan, S. ;

Schotte, F. ;

Rajer, V. S. ;

Viccaro, P. J. ;

Westferro, F. ;

Anfinrud, P. ;

Moffat, K. .

JOURNAL OF SYNCHROTRON RADIATION, 2011, 18 :658-670

[16] Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution [J].

Jordan, P ;

Fromme, P ;

Witt, HT ;

Klukas, O ;

Saenger, W ;

Krauss, N .

NATURE, 2001, 411 (6840) :909-917

[17] Structure-factor analysis of femtosecond micro-diffraction patterns from protein nanocrystals [J].

Kirian, Richard A. ;

White, Thomas A. ;

Holton, James M. ;

Chapman, Henry N. ;

Fromme, Petra ;

Barty, Anton ;

Lomb, Lukas ;

Aquila, Andrew ;

Maia, Filipe R. N. C. ;

Martin, Andrew V. ;

Fromme, Raimund ;

Wang, Xiaoyu ;

Hunter, Mark S. ;

Schmidt, Kevin E. ;

Spence, John C. H. .

ACTA CRYSTALLOGRAPHICA SECTION A, 2011, 67 :131-140

[18] X-RAY LAUE DIFFRACTION FROM PROTEIN CRYSTALS [J].

MOFFAT, K ;

SZEBENYI, D ;

BILDERBACK, D .

SCIENCE, 1984, 223 (4643) :1423-1425

[19] The frontiers of time-resolved macromolecular crystallography: movies and chirped X-ray pulses [J].

Moffat, K .

FARADAY DISCUSSIONS, 2003, 122 :65-77

[20] Semi-continuum electrostatic calculations of redox potentials in photosystem I [J].

Ptushenko, Vasily V. ;

Cherepanov, Dmitry A. ;

Krishtalik, Lev I. ;

Semenov, Alexey Yu. .

PHOTOSYNTHESIS RESEARCH, 2008, 97 (01) :55-74

← 1 2 3 →